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SLC12A6 polyclonal antibody

  • Catalog # : PAB7377
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of SLC12A6.
  • Immunogen:
  • A synthetic peptide corresponding to human SLC12A6.
  • Sequence:
  • SRHIDVCSKTKE
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 128, 122, 121, 127,
  • Reactivity:
  • Human
  • Specificity:
  • This antibody is expected to recognize all 6 reported isofoms (NP_598408.1, NP_005126.1, NP_0010.35959.1, NP_0010.35960.1, NP_0010.35961.1, NP_0010.35962.1, ).
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:16000)
    Western blot (0.1-0.3 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2-4 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • SLC12A6 polyclonal antibody (Cat # PAB7377) (0.1 ug/mL) staining of human heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • SLC12A6 polyclonal antibody (Cat # PAB7377, 2.5 ug/mL) staining of paraffin embedded human heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 9990
  • Protein Accession#:
  • NP_0010.35959.1;NP_0010.35960.1;NP_0010.35961.1;NP_0010.35962.1;NP_005126.1;NP_598408.1
  • Gene Name:
  • SLC12A6
  • Gene Alias:
  • ACCPN,DKFZp434D2135,KCC3,KCC3A,KCC3B
  • Gene Description:
  • solute carrier family 12 (potassium/chloride transporters), member 6
  • Gene Summary:
  • This gene is a member of the K-Cl cotransporter (KCC) family. K-Cl cotransporters are integral membrane proteins that lower intracellular chloride concentrations below the electrochemical equilibrium potential. The proteins encoded by this gene are activated by cell swelling induced by hypotonic conditions. Alternate splicing results in multiple transcript variants encoding different isoforms. Mutations in this gene are associated with agenesis of the corpus callosum with peripheral neuropathy. [provided by RefSeq
  • Other Designations:
  • potassium chloride cotransporter 3,potassium chloride cotransporter KCC3a-S3,solute carrier family 12, member 6
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