SLC12A6 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
SLC12A6 polyclonal antibody (Cat # PAB7377) (0.1 ug/mL) staining of human heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
SLC12A6 polyclonal antibody (Cat # PAB7377, 2.5 ug/mL) staining of paraffin embedded human heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of SLC12A6.
Immunogen
A synthetic peptide corresponding to human SLC12A6.
Sequence
SRHIDVCSKTKE
Host
Goat
Theoretical MW (kDa)
128, 122, 121, 127,
Reactivity
Human
Specificity
This antibody is expected to recognize all 6 reported isofoms (NP_598408.1, NP_005126.1, NP_0010.35959.1, NP_0010.35960.1, NP_0010.35961.1, NP_0010.35962.1, ).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:16000)
Western blot (0.1-0.3 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
SLC12A6 polyclonal antibody (Cat # PAB7377) (0.1 ug/mL) staining of human heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
SLC12A6 polyclonal antibody (Cat # PAB7377, 2.5 ug/mL) staining of paraffin embedded human heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — SLC12A6
Entrez GeneID
9990Protein Accession#
NP_0010.35959.1;NP_0010.35960.1;NP_0010.35961.1;NP_0010.35962.1;NP_005126.1;NP_598408.1Gene Name
SLC12A6
Gene Alias
ACCPN, DKFZp434D2135, KCC3, KCC3A, KCC3B
Gene Description
solute carrier family 12 (potassium/chloride transporters), member 6
Gene Ontology
HyperlinkGene Summary
This gene is a member of the K-Cl cotransporter (KCC) family. K-Cl cotransporters are integral membrane proteins that lower intracellular chloride concentrations below the electrochemical equilibrium potential. The proteins encoded by this gene are activated by cell swelling induced by hypotonic conditions. Alternate splicing results in multiple transcript variants encoding different isoforms. Mutations in this gene are associated with agenesis of the corpus callosum with peripheral neuropathy. [provided by RefSeq
Other Designations
potassium chloride cotransporter 3|potassium chloride cotransporter KCC3a-S3|solute carrier family 12, member 6
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Interactome
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Disease
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Publication Reference
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Homooligomeric and heterooligomeric associations between K+-Cl- cotransporter isoforms and between K+-Cl- and Na+-K+-Cl- cotransporters.
Simard CF, Bergeron MJ, Frenette-Cotton R, Carpentier GA, Pelchat ME, Caron L, Isenring P.
The Journal of Biological Chemistry 2007 Jun; 282(25):18083.
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Homooligomeric and heterooligomeric associations between K+-Cl- cotransporter isoforms and between K+-Cl- and Na+-K+-Cl- cotransporters.
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