MEPCE polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of MEPCE.
Immunogen
A synthetic peptide corresponding to human MEPCE.
Sequence
KQQRKFQYGNYCK
Host
Goat
Theoretical MW (kDa)
74.4
Reactivity
Human, Rat
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
MEPCE polyclonal antibody (Cat # PAB7372) (1 ug/mL) staining of rat testis lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Western Blot (Transfected lysate)
293 overexpressing MEPCE and probed with MEPCE polyclonal antibody (Cat # PAB7372) (mock transfection in first lane), tested by Origene.Enzyme-linked Immunoabsorbent Assay
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Gene Info — MEPCE
Entrez GeneID
56257Protein Accession#
NP_062552.2Gene Name
MEPCE
Gene Alias
BCDIN3, FLJ20257
Gene Description
methylphosphate capping enzyme
Omim ID
611478Gene Ontology
HyperlinkGene Summary
bicoid-interacting 3|bin3
Other Designations
bin3, bicoid-interacting 3|bin3, bicoid-interacting 3, homolog
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Interactome
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Publication Reference
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Systematic analysis of the protein interaction network for the human transcription machinery reveals the identity of the 7SK capping enzyme.
Jeronimo C, Forget D, Bouchard A, Li Q, Chua G, Poitras C, Therien C, Bergeron D, Bourassa S, Greenblatt J, Chabot B, Poirier GG, Hughes TR, Blanchette M, Price DH, Coulombe B.
Molecular Cell 2007 Jul; 27(2):262.
Application:WB-Tr, Human, HEK 293 cells.
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Systematic analysis of the protein interaction network for the human transcription machinery reveals the identity of the 7SK capping enzyme.
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