PLA2G1B polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
PLA2G1B polyclonal antibody (Cat # PAB7364) (0.01 ug/mL) staining of human pancreas lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot (Transfected lysate)
293 overexpressing PLA2G1B and probed with PLA2G1B polyclonal antibody (Cat # PAB7364) (mock transfection in first lane), tested by Origene.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of PLA2G1B.
Immunogen
A synthetic peptide corresponding to human PLA2G1B.
Sequence
NKAHKNLDTKKYCQS
Host
Goat
Theoretical MW (kDa)
16.4
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:64000)
Western Blot (0.01 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
PLA2G1B polyclonal antibody (Cat # PAB7364) (0.01 ug/mL) staining of human pancreas lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Western Blot (Transfected lysate)
293 overexpressing PLA2G1B and probed with PLA2G1B polyclonal antibody (Cat # PAB7364) (mock transfection in first lane), tested by Origene.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PLA2G1B
Entrez GeneID
5319Protein Accession#
NP_000919.1Gene Name
PLA2G1B
Gene Alias
MGC119834, MGC119835, PLA2, PLA2A, PPLA2
Gene Description
phospholipase A2, group IB (pancreas)
Omim ID
172410Gene Ontology
HyperlinkGene Summary
Phospholipase A2 (EC 3.1.1.4) catalyzes the release of fatty acids from glycero-3-phosphocholines. The best known varieties are the digestive enzymes secreted as zymogens by the pancreas of mammals. Sequences of pancreatic PLA2 enzymes from a variety of mammals have been reported. One striking feature of these enzymes is their close homology to venom phospholipases of snakes. Other forms of PLA2 have been isolated from brain, liver, lung, spleen, intestine, macrophages, leukocytes, erythrocytes, inflammatory exudates, chondrocytes, and platelets (Seilhamer et al., 1986 [PubMed 3028739]) .[supplied by OMIM
Other Designations
phospholipase A2, group IB
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Interactome
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Pathway
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Disease
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Publication Reference
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Expression and induction of secretory phospholipase A2 group IB in brain.
Kolko M, Christoffersen NR, Varoqui H, Bazan NG.
Cellular and Molecular Neurobiology 2005 Nov; 25(7):1107.
Application：WB-Ti, Rat, Monkey, Brain, COS7 cells.
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Expression and induction of secretory phospholipase A2 group IB in brain.
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