HTATSF1 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of HTATSF1.
Immunogen
A synthetic peptide corresponding to human HTATSF1.
Sequence
C-QELYGDGKDGDTQTD
Host
Goat
Theoretical MW (kDa)
85.9
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:16000)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
HTATSF1 polyclonal antibody (Cat # PAB7353) (1 ug/mL) staining of Daudi cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — HTATSF1
Entrez GeneID
27336Protein Accession#
NP_055315.2Gene Name
HTATSF1
Gene Alias
TAT-SF1, dJ196E23.2
Gene Description
HIV-1 Tat specific factor 1
Omim ID
300346Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene functions as a cofactor for the stimulation of transcriptional elongation by HIV-1 Tat, which binds to the HIV-1 promoter through Tat-TAR interaction. This protein may also serve as a dual-function factor to couple transcription and splicing and to facilitate their reciprocal activation. Alternatively spliced transcript variants have been found for this gene
Other Designations
HIV TAT specific factor 1|OTTHUMP00000024126|cofactor required for Tat activation of HIV-1 transcription
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Interactome
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Disease
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Publication Reference
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Coordination of transcription factor phosphorylation and histone methylation by the P-TEFb kinase during human immunodeficiency virus type 1 transcription.
Zhou M, Deng L, Lacoste V, Park HU, Pumfery A, Kashanchi F, Brady JN, Kumar A.
Journal of Virology 2004 Dec; 78(24):13522.
Application:ChIP, Human, OM10.1 cells.
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Coordination of transcription factor phosphorylation and histone methylation by the P-TEFb kinase during human immunodeficiency virus type 1 transcription.
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