SMEK1 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of SMEK1.
Immunogen
A synthetic peptide corresponding to human SMEK1.
Sequence
C-EQQRERQDNPKLD
Host
Goat
Theoretical MW (kDa)
93.9
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:16000)
Western Blot (0.3-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
SMEK1 polyclonal antibody (Cat # PAB7349) (0.3 ug/mL) staining of human pancreas lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Western Blot (Transfected lysate)
293 overexpressing SMEK1 and probed with SMEK1 polyclonal antibody (Cat # PAB7349) (mock transfection in first lane), tested by Origene.Enzyme-linked Immunoabsorbent Assay
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Gene Info — SMEK1
Entrez GeneID
55671Protein Accession#
NP_115949.3Gene Name
SMEK1
Gene Alias
FLFL1, KIAA2010, MSTP033, PP4R3A, smk-1, smk1
Gene Description
SMEK homolog 1, suppressor of mek1 (Dictyostelium)
Omim ID
610351Gene Ontology
HyperlinkGene Summary
O
Other Designations
SMEK homolog 1, suppressor of mek1
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Interactome
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Publication Reference
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PHA-4/Foxa mediates diet-restriction-induced longevity of C. elegans.
Panowski SH, Wolff S, Aguilaniu H, Durieux J, Dillin A.
Nature 2007 May; 447(7144):550.
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PHA-4/Foxa mediates diet-restriction-induced longevity of C. elegans.
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