STEAP4 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of STEAP4.
Immunogen
A synthetic peptide corresponding to human STEAP4.
Sequence
CVDNTLTRIRQGWERN
Host
Goat
Theoretical MW (kDa)
52
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:4000)
Western Blot (0.3-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
STEAP4 polyclonal antibody (Cat # PAB7348) (0.3 ug/mL) staining of human adipose lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Western Blot (Transfected lysate)
293 overexpressing STEAP4 and probed with STEAP4 polyclonal antibody (Cat # PAB7348) (mock transfection in first lane), tested by Origene.Enzyme-linked Immunoabsorbent Assay
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Gene Info — STEAP4
Entrez GeneID
79689Protein Accession#
NP_078912.2Gene Name
STEAP4
Gene Alias
DKFZp666D049, FLJ23153, STAMP2, TIARP, TNFAIP9
Gene Description
STEAP family member 4
Omim ID
611098Gene Ontology
HyperlinkGene Summary
alpha-induced protein 9|tumor necrosis-alpha-induced adipose-related protein
Other Designations
six transmembrane prostate protein 2|tumor necrosis factor, alpha-induced protein 9|tumor necrosis-alpha-induced adipose-related protein
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Interactome
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Disease
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Publication Reference
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Coordinated regulation of nutrient and inflammatory responses by STAMP2 is essential for metabolic homeostasis.
Wellen KE, Fucho R, Gregor MF, Furuhashi M, Morgan C, Lindstad T, Vaillancourt E, Gorgun CZ, Saatcioglu F, Hotamisligil GS.
Cell 2007 May; 129(3):537.
Application:IHC, WB, Mouse, Mouse adipose-tissue.
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Coordinated regulation of nutrient and inflammatory responses by STAMP2 is essential for metabolic homeostasis.
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