PCBP4 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
PCBP4 polyclonal antibody (Cat # PAB7325) (0.03 ug/mL) staining of HeLa cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot (Transfected lysate)
293 overexpressing PCBP4 and probed with PCBP4 polyclonal antibody (Cat # PAB7325) (mock transfection in first lane), tested by Origene.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of PCBP4.
Immunogen
A synthetic peptide corresponding to human PCBP4.
Sequence
C-NGSKKAERQKFSPY
Host
Goat
Theoretical MW (kDa)
43.9, 37.9, 41.5, 41
Reactivity
Human
Specificity
This antibody is expected to recognize all reported isoforms (NP_065151.1, NP_127501.1, NP_127502.1 and NP_12750.3.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:128000)
Western Blot (1~3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
PCBP4 polyclonal antibody (Cat # PAB7325) (0.03 ug/mL) staining of HeLa cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Western Blot (Transfected lysate)
293 overexpressing PCBP4 and probed with PCBP4 polyclonal antibody (Cat # PAB7325) (mock transfection in first lane), tested by Origene.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PCBP4
Entrez GeneID
57060Protein Accession#
NP_065151.1;NP_127501.1;NP_127502.1;NP_12750.3.1Gene Name
PCBP4
Gene Alias
LIP4, MCG10
Gene Description
poly(rC) binding protein 4
Omim ID
608503Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the KH-domain protein subfamily. Proteins of this subfamily, also referred to as alpha-CPs, bind to RNA with a specificity for C-rich pyrimidine regions. Alpha-CPs play important roles in post-transcriptional activities and have different cellular distributions. This gene is induced by the p53 tumor suppressor, and the encoded protein can suppress cell proliferation by inducing apoptosis and cell cycle arrest in G(2)-M. This gene's protein is found in the cytoplasm, yet it lacks the nuclear localization signals found in other subfamily members. Multiple alternatively spliced transcript variants have been described, but the full-length nature for only some has been determined. [provided by RefSeq
Other Designations
LYST-interacting protein|RNA binding protein MCG10|alphaCP-4 protein
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Interactome
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Disease
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Publication Reference
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Alpha CP-4, encoded by a putative tumor suppressor gene at 3p21, but not its alternative splice variant alpha CP-4a, is underexpressed in lung cancer.
Pio R, Zudaire I, Pino I, Castano Z, Zabalegui N, Vicent S, Garcia-Amigot F, Odero MD, Lozano MD, Garcia-Foncillas J, Calasanz MJ, Montuenga LM.
Cancer Research 2004 Jun; 64(12):4171.
Application：IHC-P, Human, Human lung cancer.
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Alpha CP-4, encoded by a putative tumor suppressor gene at 3p21, but not its alternative splice variant alpha CP-4a, is underexpressed in lung cancer.
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