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Last updated: 2016/11/27
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PCBP4 polyclonal antibody

  • Catalog # : PAB7325
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of PCBP4.
  • Immunogen:
  • A synthetic peptide corresponding to human PCBP4.
  • Sequence:
  • C-NGSKKAERQKFSPY
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 43.9, 37.9, 41.5, 41
  • Reactivity:
  • Human
  • Specificity:
  • This antibody is expected to recognize all reported isoforms (NP_065151.1, NP_127501.1, NP_127502.1 and NP_12750.3.1).
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:128000)
    Western Blot (1~3 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • PCBP4 polyclonal antibody (Cat # PAB7325) (0.03 ug/mL) staining of HeLa cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • 293 overexpressing PCBP4 and probed with PCBP4 polyclonal antibody (Cat # PAB7325) (mock transfection in first lane), tested by Origene.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Protein Accession#:
  • NP_065151.1;NP_127501.1;NP_127502.1;NP_12750.3.1
  • Gene Name:
  • PCBP4
  • Gene Alias:
  • LIP4,MCG10
  • Gene Description:
  • poly(rC) binding protein 4
  • Gene Summary:
  • This gene encodes a member of the KH-domain protein subfamily. Proteins of this subfamily, also referred to as alpha-CPs, bind to RNA with a specificity for C-rich pyrimidine regions. Alpha-CPs play important roles in post-transcriptional activities and have different cellular distributions. This gene is induced by the p53 tumor suppressor, and the encoded protein can suppress cell proliferation by inducing apoptosis and cell cycle arrest in G(2)-M. This gene's protein is found in the cytoplasm, yet it lacks the nuclear localization signals found in other subfamily members. Multiple alternatively spliced transcript variants have been described, but the full-length nature for only some has been determined. [provided by RefSeq
  • Other Designations:
  • LYST-interacting protein,RNA binding protein MCG10,alphaCP-4 protein
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