NEDD9 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
NEDD9 polyclonal antibody (Cat # PAB7323) (0.1 ug/mL) staining of human kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of NEDD9.
Immunogen
A synthetic peptide corresponding to human NEDD9.
Sequence
NKPQNKCDDLDR
Host
Goat
Theoretical MW (kDa)
92.9
Reactivity
Human
Specificity
This antibody is expected to recognize isoform 1 (NP_006394.1) only.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:8000)
Western Blot (0.1-0.3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
NEDD9 polyclonal antibody (Cat # PAB7323) (0.1 ug/mL) staining of human kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — NEDD9
Entrez GeneID
4739Protein Accession#
NP_006394.1;NP_892011.2Gene Name
NEDD9
Gene Alias
CAS-L, CAS2, CASL, CASS2, HEF1, dJ49G10.2, dJ761I2.1
Gene Description
neural precursor cell expressed, developmentally down-regulated 9
Omim ID
602265Gene Ontology
HyperlinkGene Summary
O
Other Designations
Cas scaffolding protein family member 2|Crk-associated substrate related|OTTHUMP00000016030|cas-like docking|dJ49G10.2 (Enhancer of Filamentation 1 (HEF1))|dJ761I2.1 (enhancer of filamentation (HEF1))|enhancer of filamentation 1
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Interactome
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Disease
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Publication Reference
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Comparative oncogenomics identifies NEDD9 as a melanoma metastasis gene.
Kim M, Gans JD, Nogueira C, Wang A, Paik JH, Feng B, Brennan C, Hahn WC, Cordon-Cardo C, Wagner SN, Flotte TJ, Duncan LM, Granter SR, Chin L.
Cell 2006 Jun; 125(7):1269.
Application：IF, IP, IHC-P, WB-Tr, Human, Human melanoma tissues, Malme3M, MK24, UACC257 cells.
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Comparative oncogenomics identifies NEDD9 as a melanoma metastasis gene.
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