SAR1B polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
SAR1B polyclonal antibody (Cat # PAB7320) (0.03 ug/mL) staining of mouse liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot (Transfected lysate)
293 overexpressing SAR1B and probed with SAR1B polyclonal antibody (Cat # PAB7320) (mock transfection in first lane), tested by Origene.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of SAR1B.
Immunogen
A synthetic peptide corresponding to human SAR1B.
Sequence
C-DHERLLESKEELD
Host
Goat
Theoretical MW (kDa)
22.4, 22.4
Reactivity
Mouse, Rat
Specificity
Both reported variants (NP_001028675.1 and NP_057187.1) represent identical protein.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:8000)
Western Blot (0.03-0.1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
SAR1B polyclonal antibody (Cat # PAB7320) (0.03 ug/mL) staining of mouse liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Western Blot (Transfected lysate)
293 overexpressing SAR1B and probed with SAR1B polyclonal antibody (Cat # PAB7320) (mock transfection in first lane), tested by Origene.Enzyme-linked Immunoabsorbent Assay
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Gene Info — SAR1B
Entrez GeneID
51128Protein Accession#
NP_001028675.1;NP_057187.1Gene Name
SAR1B
Gene Alias
CMRD, GTBPB, SARA2
Gene Description
SAR1 homolog B (S. cerevisiae)
Gene Ontology
HyperlinkOther Designations
2310075M17Rik|GTP-binding protein Sara|SAR1 gene homolog B|SAR1a gene homolog 2
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Interactome
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Publication Reference
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Tissue-specific stable isotope measurements of postprandial lipid metabolism in familial combined hyperlipidaemia.
Evans K, Burdge GC, Wootton SA, Collins JM, Clark ML, Tan GD, Karpe F, Frayn KN.
Atherosclerosis 2008 Mar; 197(1):164.
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Tissue-specific stable isotope measurements of postprandial lipid metabolism in familial combined hyperlipidaemia.
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