NCF4 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
NCF4 polyclonal antibody (Cat # PAB7315) (0.5 ug/mL) staining of Daudi cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot (Transfected lysate)
293 overexpressing NCF4 and probed with NCF4 polyclonal antibody (Cat # PAB7315) (mock transfection in first lane), tested by Origene.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
NCF4 polyclonal antibody (Cat # PAB7315) (2.5 ug/mL) staining of paraffin embedded human tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of NCF4.
Immunogen
A synthetic peptide corresponding to human NCF4.
Sequence
C-KDFPEEDDPTN
Host
Goat
Theoretical MW (kDa)
39.0, 38.8
Reactivity
Human
Specificity
This antibody is expected to recognize both reported isoforms (NP_000622.2 and NP_0.38202.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western blot (0.3-1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
NCF4 polyclonal antibody (Cat # PAB7315) (0.5 ug/mL) staining of Daudi cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Western Blot (Transfected lysate)
293 overexpressing NCF4 and probed with NCF4 polyclonal antibody (Cat # PAB7315) (mock transfection in first lane), tested by Origene.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
NCF4 polyclonal antibody (Cat # PAB7315) (2.5 ug/mL) staining of paraffin embedded human tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — NCF4
Entrez GeneID
4689Protein Accession#
NP_000622.2;NP_0.38202.1Gene Name
NCF4
Gene Alias
MGC3810, NCF, P40PHOX, SH3PXD4
Gene Description
neutrophil cytosolic factor 4, 40kDa
Omim ID
601488Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq
Other Designations
OTTHUMP00000028736|OTTHUMP00000043756|neutrophil NADPH oxidase factor 4|neutrophil cytosol factor 4|neutrophil cytosolic factor 4
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Interactome
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Pathway
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Disease
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Publication Reference
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Genome-wide association study identifies new susceptibility loci for Crohn disease and implicates autophagy in disease pathogenesis.
Rioux JD, Xavier RJ, Taylor KD, Silverberg MS, Goyette P, Huett A, Green T, Kuballa P, Barmada MM, Datta LW, Shugart YY, Griffiths AM, Targan SR, Ippoliti AF, Bernard EJ, Mei L, Nicolae DL, Regueiro M, Schumm LP, Steinhart AH, Rotter JI, Duerr RH, Cho JH, Daly MJ, Brant SR.
Nature Genetics 2007 May; 39(5):596.
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Genome-wide association study identifies new susceptibility loci for Crohn disease and implicates autophagy in disease pathogenesis.
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