FH polyclonal antibody
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More Files
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of FH.
Immunogen
A synthetic peptide corresponding to human FH.
Sequence
C-HPNDHVNKSQSSND
Host
Goat
Theoretical MW (kDa)
54.6
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:64000)
Western Blot (0.01-0.03 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
FH polyclonal antibody (Cat # PAB7310) (0.01 ug/mL) staining of human kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Western Blot (Transfected lysate)
293 overexpressing FH and probed with FH polyclonal antibody (Cat # PAB7310) (mock transfection in first lane), tested by Origene.Enzyme-linked Immunoabsorbent Assay
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Gene Info — FH
Entrez GeneID
2271Protein Accession#
NP_000134.2Gene Name
FH
Gene Alias
HLRCC, LRCC, MCL, MCUL1
Gene Description
fumarate hydratase
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy. [provided by RefSeq
Other Designations
OTTHUMP00000037573|fumarase|multiple hereditary cutaneous leiomyomata
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Interactome
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Pathway
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Disease
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Publication Reference
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Alterations in the mitochondrial proteome of neuroblastoma cells in response to complex 1 inhibition.
Burtee F, De Girolamo LA, Hargreaves AJ, Billett EE.
Journal of Proteome Research 2011 Apr; 10(4):1974.
Application:WB-Ce, Mouse, Mouse N2a neuroblastoma cells.
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Targeted inactivation of fh1 causes proliferative renal cyst development and activation of the hypoxia pathway.
Pollard PJ, Spencer-Dene B, Shukla D, Howarth K, Nye E, El-Bahrawy M, Deheragoda M, Joannou M, McDonald S, Martin A, Igarashi P, Varsani-Brown S, Rosewell I, Poulsom R, Maxwell P, Stamp GW, Tomlinson IP.
Cancer Cell 2007 Apr; 11(4):311.
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Alterations in the mitochondrial proteome of neuroblastoma cells in response to complex 1 inhibition.
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