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Last updated: 2017/3/26
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FH polyclonal antibody

  • Catalog # : PAB7310
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of FH.
  • Immunogen:
  • A synthetic peptide corresponding to human FH.
  • Sequence:
  • C-HPNDHVNKSQSSND
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 54.6
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:64000)
    Western Blot (0.01-0.03 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • FH polyclonal antibody (Cat # PAB7310) (0.01 ug/mL) staining of human kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • 293 overexpressing FH and probed with FH polyclonal antibody (Cat # PAB7310) (mock transfection in first lane), tested by Origene.
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 2271
  • Gene Name:
  • FH
  • Gene Alias:
  • HLRCC,LRCC,MCL,MCUL1
  • Gene Description:
  • fumarate hydratase
  • Gene Summary:
  • The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000037573,fumarase,multiple hereditary cutaneous leiomyomata
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