RPL8 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
RPL8 polyclonal antibody (Cat # PAB7307) (0.01 ug/mL) staining of HepG2 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
RPL8 polyclonal antibody (Cat # PAB7307) (2.5 ug/mL) staining of paraffin embedded human cerebral cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of RPL8.
Immunogen
A synthetic peptide corresponding to human RPL8.
Sequence
KAGRAYHKYKAKRNC
Host
Goat
Theoretical MW (kDa)
28
Reactivity
Human
Specificity
The reported variants represent identical protein (NP_000964.1 and NP_150644.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:128000)
Western blot (0.01-0.03 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
RPL8 polyclonal antibody (Cat # PAB7307) (0.01 ug/mL) staining of HepG2 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
RPL8 polyclonal antibody (Cat # PAB7307) (2.5 ug/mL) staining of paraffin embedded human cerebral cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — RPL8
Entrez GeneID
6132Protein Accession#
NP_000964.1;NP_150644.1Gene Name
RPL8
Gene Alias
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Gene Description
ribosomal protein L8
Omim ID
604177Gene Ontology
HyperlinkGene Summary
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L2P family of ribosomal proteins. It is located in the cytoplasm. In rat, the protein associates with the 5.8S rRNA, very likely participates in the binding of aminoacyl-tRNA, and is a constituent of the elongation factor 2-binding site at the ribosomal subunit interface. Alternatively spliced transcript variants encoding the same protein exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq
Other Designations
60S ribosomal protein L8
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Interactome
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Pathway
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Publication Reference
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Shared MHC class II-dependent melanoma ribosomal protein L8 identified by phage display.
Swoboda RK, Somasundaram R, Caputo L, Ochoa EM, Gimotty PA, Marincola FM, Van Belle P, Barth S, Elder D, Guerry D, Czerniecki B, Schuchter L, Vonderheide RH, Herlyn D.
Cancer Research 2007 Apr; 67(8):3555.
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Shared MHC class II-dependent melanoma ribosomal protein L8 identified by phage display.
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