Il12b polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
The Il12b polyclonal antibody (Cat # PAB7306) (1 ug/mL) staining of rat liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of Il12b.
Immunogen
A synthetic peptide corresponding to mouse Il12b.
Sequence
C-EARQQNKYENYS
Host
Goat
Theoretical MW (kDa)
38.2
Reactivity
Mouse, Rat
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:4000)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
The Il12b polyclonal antibody (Cat # PAB7306) (1 ug/mL) staining of rat liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — Il12b
Entrez GeneID
16160Protein Accession#
NP_0.32378.1Gene Name
Il12b
Gene Alias
Il-12b, Il-12p40, Il12p40, p40
Gene Description
interleukin 12b
Gene Ontology
HyperlinkOther Designations
IL-12 p40|IL-23 subunit p40|OTTMUSP00000005619
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Publication Reference
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Distinct roles for IL-6 and IL-12p40 in mediating protection against Leishmania donovani and the expansion of IL-10+ CD4+ T cells.
Stager S, Maroof A, Zubairi S, Sanos SL, Kopf M, Kaye PM.
European Journal of Immunology 2006 Jul; 36(7):1764.
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Distinct roles for IL-6 and IL-12p40 in mediating protection against Leishmania donovani and the expansion of IL-10+ CD4+ T cells.
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