G6PD polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of G6PD.
Immunogen
A synthetic peptide corresponding to amino acids 308-320 of human G6PD.
Sequence
C-STNSDDVRDEKVK
Host
Goat
Theoretical MW (kDa)
62.5, 59.3
Reactivity
Human
Specificity
This antibody is expected to recognize both reported isoforms (NP_00.0393.4 and NP_0010.35810.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Sandwich ELISA (5-10 ug/mL)
Western blot (0.03-0.1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
G6PD polyclonal antibody (Cat # PAB7299) (0.03 ug/mL) staining of human testis lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
G6PD polyclonal antibody (Cat # PAB7299, 2.5 ug/mL) staining of paraffin embedded human testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
Sandwich ELISA
G6PD polyclonal antibody (Cat # PAB7299) (5 ug/mL) as the reporter with the capture rabbit antibody (5 ug/mL). -
Gene Info — G6PD
Entrez GeneID
2539Protein Accession#
NP_00.0393.4;NP_0010.35810.1Gene Name
G6PD
Gene Alias
G6PD1
Gene Description
glucose-6-phosphate dehydrogenase
Omim ID
305900Gene Ontology
HyperlinkGene Summary
This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000026034|glucose-6-phosphate 1-dehydrogenase|glucose-6-phosphate dehydrogenase, G6PD
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Interactome
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Pathway
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Disease
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Publication Reference
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X-linked G6PD deficiency protects hemizygous males but not heterozygous females against severe malaria.
Guindo A, Fairhurst RM, Doumbo OK, Wellems TE, Diallo DA.
PLoS medicine 2007 Mar; 4(3):e66.
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X-linked G6PD deficiency protects hemizygous males but not heterozygous females against severe malaria.
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