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Last updated: 2017/6/18
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G6PD polyclonal antibody

  • Catalog # : PAB7299
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of G6PD.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 308-320 of human G6PD.
  • Sequence:
  • C-STNSDDVRDEKVK
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 62.5, 59.3
  • Reactivity:
  • Human
  • Specificity:
  • This antibody is expected to recognize both reported isoforms (NP_00.0393.4 and NP_0010.35810.1).
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Recommend Usage:
  • ELISA (1:32000)
    Sandwich ELISA (5-10 ug/mL)
    Western blot (0.03-0.1 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2-4 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • G6PD polyclonal antibody (Cat # PAB7299) (0.03 ug/mL) staining of human testis lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • G6PD polyclonal antibody (Cat # PAB7299, 2.5 ug/mL) staining of paraffin embedded human testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
  • ELISA
  • Sandwich ELISA
  • Sandwich ELISA
  • G6PD polyclonal antibody (Cat # PAB7299) (5 ug/mL) as the reporter with the capture rabbit antibody (5 ug/mL).
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 2539
  • Protein Accession#:
  • NP_00.0393.4;NP_0010.35810.1
  • Gene Name:
  • G6PD
  • Gene Alias:
  • G6PD1
  • Gene Description:
  • glucose-6-phosphate dehydrogenase
  • Gene Summary:
  • This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000026034,glucose-6-phosphate 1-dehydrogenase,glucose-6-phosphate dehydrogenase, G6PD
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