NCAPH polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
NCAPH polyclonal antibody (Cat # PAB7285) (0.01 ug/mL) staining of K-562 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of NCAPH.
Immunogen
A synthetic peptide corresponding to human NCAPH.
Sequence
C-TEDLSDVLVRQGD
Host
Goat
Theoretical MW (kDa)
82.5
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:128000)
Western Blot (0.01-0.03 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
NCAPH polyclonal antibody (Cat # PAB7285) (0.01 ug/mL) staining of K-562 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — NCAPH
Entrez GeneID
23397Protein Accession#
NP_056156.2Gene Name
NCAPH
Gene Alias
BRRN1, CAP-H, HCAP-H
Gene Description
non-SMC condensin I complex, subunit H
Omim ID
602332Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the barr gene family and a regulatory subunit of the condensin complex. This complex is required for the conversion of interphase chromatin into condensed chromosomes. The protein encoded by this gene is associated with mitotic chromosomes, except during the early phase of chromosome condensation. During interphase, the protein has a distinct punctate nucleolar localization. [provided by RefSeq
Other Designations
XCAP-H homolog|barren homolog 1|chromosome-associated protein H|condensin complex subunit 2
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Interactome
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Publication Reference
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Anaphase onset before complete DNA replication with intact checkpoint responses.
Torres-Rosell J, De Piccoli G, Cordon-Preciado V, Farmer S, Jarmuz A, Machin F, Pasero P, Lisby M, Haber JE, Aragon L.
Science 2007 Mar; 315(5817):1411.
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Anaphase onset before complete DNA replication with intact checkpoint responses.
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