NR0B1 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of NR0B1.
Immunogen
A synthetic peptide corresponding to human NR0B1.
Sequence
CGEDHPQQGSTLY
Host
Goat
Theoretical MW (kDa)
51.7
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western Blot (0.5-1.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
NR0B1 polyclonal antibody (Cat # PAB7276) (0.5 ug/mL) staining of human testis lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — NR0B1
Entrez GeneID
190Protein Accession#
NP_000466.2Gene Name
NR0B1
Gene Alias
AHC, AHCH, AHX, DAX-1, DAX1, DSS, GTD, HHG, NROB1
Gene Description
nuclear receptor subfamily 0, group B, member 1
Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that contains a DNA-binding domain. The encoded protein acts as a dominant-negative regulator of transcription which is mediated by the retinoic acid receptor. This protein also functions as an anti-testis gene by acting antagonistically to Sry. Mutations in this gene result in both X-linked congenital adrenal hypoplasia and hypogonadotropic hypogonadism. [provided by RefSeq
Other Designations
OTTHUMP00000023102|gonadotropin deficiency|nuclear hormone receptor
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Interactome
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Publication Reference
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A protein interaction network for pluripotency of embryonic stem cells.
Wang J, Rao S, Chu J, Shen X, Levasseur DN, Theunissen TW, Orkin SH.
Nature 2006 Nov; 444(7117):364.
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A protein interaction network for pluripotency of embryonic stem cells.
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