PAX6 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
PAX6 polyclonal antibody (Cat # PAB7271) (0.3 ug/mL) staining of U251 (1), (0.03 ug/mL) nuclear K562 (2) and (1 ug/mL) nuclear HeLa (3) cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Flow Cytometry
PAX6 polyclonal antibody (Cat # PAB7271) Flow cytometric analysis of paraformaldehyde fixed U251 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of PAX6.
Immunogen
A synthetic peptide corresponding to human PAX6.
Sequence
C-REEKLRNQRRQASN
Host
Goat
Theoretical MW (kDa)
46.7, 48.2
Reactivity
Mouse
Specificity
This antibody is expected to recognize both reported isoforms (NP_000271.1 and NP_001595.2).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western Blot (0.3-1 ug/mL)
Flow Cytometry (10 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
PAX6 polyclonal antibody (Cat # PAB7271) (0.3 ug/mL) staining of U251 (1), (0.03 ug/mL) nuclear K562 (2) and (1 ug/mL) nuclear HeLa (3) cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
PAX6 polyclonal antibody (Cat # PAB7271) Flow cytometric analysis of paraformaldehyde fixed U251 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody. -
Gene Info — PAX6
Entrez GeneID
5080Protein Accession#
NP_000271.1;NP_001595.2Gene Name
PAX6
Gene Alias
AN, AN2, D11S812E, MGC17209, MGDA, WAGR
Gene Description
paired box 6
Gene Ontology
HyperlinkGene Summary
This gene encodes paired box gene 6, one of many human homologs of the Drosophila melanogaster gene prd. In addition to the hallmark feature of this gene family, a conserved paired box domain, the encoded protein also contains a homeo box domain. Both domains are known to bind DNA, and function as regulators of gene transcription. This gene is expressed in the developing nervous system, and in developing eyes. Mutations in this gene are known to cause ocular disorders such as aniridia and Peter's anomaly. Alternatively spliced transcript variants encoding either the same or different isoform have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000038833|OTTHUMP00000038834|OTTHUMP00000038835|OTTHUMP00000038836|OTTHUMP00000038837|OTTHUMP00000038838|OTTHUMP00000038839|OTTHUMP00000038840|paired box gene 6|paired box homeotic gene-6
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Interactome
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Pathway
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Disease
- Abnormalities
- Alcoholism
- Atherosclerosis
- Atrophy
- Autistic Disorder
- Blepharoptosis
- Calcinosis
- Cleft Lip
- Cleft Palate
+ View More Disease
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Publication Reference
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Neural stem/progenitor cells express 20 tenascin C isoforms that are differentially regulated by Pax6.
von Holst A, Egbers U, Prochiantz A, Faissner A.
The Journal of Biological Chemistry 2007 Mar; 282(12):9172.
Application：IF, IHC-Fr, Mouse, E13 MGE cell.
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Neural stem/progenitor cells express 20 tenascin C isoforms that are differentially regulated by Pax6.
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