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Last updated: 2022/7/3
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PAX6 polyclonal antibody 

  • Catalog # : PAB7271
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of PAX6.
  • Immunogen:
  • A synthetic peptide corresponding to human PAX6.
  • Sequence:
  • C-REEKLRNQRRQASN
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 46.7, 48.2
  • Reactivity:
  • Mouse
  • Specificity:
  • This antibody is expected to recognize both reported isoforms (NP_000271.1 and NP_001595.2).
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:32000)
    Western Blot (0.3-1 ug/mL)
    Flow Cytometry (10 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • PAX6 polyclonal antibody (Cat # PAB7271) (0.3 ug/mL) staining of U251 (1), (0.03 ug/mL) nuclear K562 (2) and (1 ug/mL) nuclear HeLa (3) cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
  • Enzyme-linked Immunoabsorbent Assay
  • Flow Cytometry
  • Flow Cytometry
  • PAX6 polyclonal antibody (Cat # PAB7271) Flow cytometric analysis of paraformaldehyde fixed U251 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Enzyme-linked Immunoabsorbent Assay
  • Gene Information
  • Entrez GeneID:
  • 5080
  • Protein Accession#:
  • NP_000271.1;NP_001595.2
  • Gene Name:
  • PAX6
  • Gene Alias:
  • AN,AN2,D11S812E,MGC17209,MGDA,WAGR
  • Gene Description:
  • paired box 6
  • Gene Summary:
  • This gene encodes paired box gene 6, one of many human homologs of the Drosophila melanogaster gene prd. In addition to the hallmark feature of this gene family, a conserved paired box domain, the encoded protein also contains a homeo box domain. Both domains are known to bind DNA, and function as regulators of gene transcription. This gene is expressed in the developing nervous system, and in developing eyes. Mutations in this gene are known to cause ocular disorders such as aniridia and Peter's anomaly. Alternatively spliced transcript variants encoding either the same or different isoform have been found for this gene. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000038833,OTTHUMP00000038834,OTTHUMP00000038835,OTTHUMP00000038836,OTTHUMP00000038837,OTTHUMP00000038838,OTTHUMP00000038839,OTTHUMP00000038840,paired box gene 6,paired box homeotic gene-6
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