PAX6 polyclonal antibody (Cat # PAB7271) (0.3 ug/mL) staining of U251 (1), (0.03 ug/mL) nuclear K562 (2) and (1 ug/mL) nuclear HeLa (3) cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
PAX6 polyclonal antibody (Cat # PAB7271) Flow cytometric analysis of paraformaldehyde fixed U251 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
This gene encodes paired box gene 6, one of many human homologs of the Drosophila melanogaster gene prd. In addition to the hallmark feature of this gene family, a conserved paired box domain, the encoded protein also contains a homeo box domain. Both domains are known to bind DNA, and function as regulators of gene transcription. This gene is expressed in the developing nervous system, and in developing eyes. Mutations in this gene are known to cause ocular disorders such as aniridia and Peter's anomaly. Alternatively spliced transcript variants encoding either the same or different isoform have been found for this gene. [provided by RefSeq