FABP2 polyclonal antibody
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More Files
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of FABP2.
Immunogen
A synthetic peptide corresponding to C-terminus of human FABP2.
Sequence
C-EGVEAKRIFKKD
Host
Goat
Theoretical MW (kDa)
15.2
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:64000)
Flow Cytometry (10 ug/mL)
Western blot (0.001 - 0.003 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
FABP2 polyclonal antibody (Cat # PAB7258) (0.001 ug/mL) staining of human duodenum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Western Blot (Transfected lysate)
293 overexpressing FABP2 and probed with FABP2 polyclonal antibody (Cat # PAB7258) (mock transfection in first lane), tested by Origene.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
FABP2 polyclonal antibody (Cat # PAB7258) (2.5 ug/mL) staining of paraffin embedded human small intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
FABP2 polyclonal antibody (Cat # PAB7258) Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody. -
Gene Info — FABP2
Entrez GeneID
2169Protein Accession#
NP_000125.2Gene Name
FABP2
Gene Alias
FABPI, I-FABP, MGC133132
Gene Description
fatty acid binding protein 2, intestinal
Omim ID
134640Gene Ontology
HyperlinkGene Summary
The intracellular fatty acid-binding proteins (FABPs) belong to a multigene family with nearly twenty identified members. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism and/or transport of long-chain fatty acids. They may also be responsible in the modulation of cell growth and proliferation. Intestinal fatty acid-binding protein 2 gene contains four exons and is an abundant cytosolic protein in small intestine epithelial cells. This gene has a polymorphism at codon 54 that identified an alanine-encoding allele and a threonine-encoding allele. Thr-54 protein is associated with increased fat oxidation and insulin resistance. [provided by RefSeq
Other Designations
Fatty acid-binding protein, intestinal|intestinal fatty acid binding protein 2
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Interactome
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Pathway
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Disease
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Publication Reference
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Thr54 allele of the FABP2 gene affects resting metabolic rate and visceral obesity.
Takakura Y, Yoshioka K, Umekawa T, Kogure A, Toda H, Yoshikawa T, Yoshida T.
Diabetes Research and Clinical Practice 2005 Jan; 67(1):36.
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Thr54 allele of the FABP2 gene affects resting metabolic rate and visceral obesity.
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