TIA1 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
TIA1 polyclonal antibody (Cat # PAB7257) (0.1 ug/mL) staining of Jurkat cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of TIA1.
Immunogen
A synthetic peptide corresponding to human TIA1.
Sequence
C-PKSTYESNTKQ
Host
Goat
Theoretical MW (kDa)
41.8, 43
Reactivity
Human
Specificity
This antibody is expected to recognize both reported isoforms (NP_071320.1 and NP_071505.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:128000)
Western Blot (0.1-0.3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
TIA1 polyclonal antibody (Cat # PAB7257) (0.1 ug/mL) staining of Jurkat cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — TIA1
Entrez GeneID
7072Protein Accession#
NP_071320.1;NP_071505.1Gene Name
TIA1
Gene Alias
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Gene Description
TIA1 cytotoxic granule-associated RNA binding protein
Omim ID
603518Gene Ontology
HyperlinkGene Summary
The product encoded by this gene is a member of a RNA-binding protein family and possesses nucleolytic activity against cytotoxic lymphocyte (CTL) target cells. It has been suggested that this protein may be involved in the induction of apoptosis as it preferentially recognizes poly(A) homopolymers and induces DNA fragmentation in CTL targets. The major granule-associated species is a 15-kDa protein that is thought to be derived from the carboxyl terminus of the 40-kDa product by proteolytic processing. Alternative splicing resulting in different isoforms of this gene product has been described in the literature. [provided by RefSeq
Other Designations
T-cell-restricted intracellular antigen-1|TIA1 cytotoxic granule-associated RNA-binding protein|TIA1 protein|cytotoxic granule-associated RNA-binding protein|nucleolysin TIA-1 isoform p40|p40-TIA-1 (containing p15-TIA-1)
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Interactome
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Publication Reference
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Polysome-bound endonuclease PMR1 is targeted to stress granules via stress-specific binding to TIA-1.
Yang F, Peng Y, Murray EL, Otsuka Y, Kedersha N, Schoenberg DR.
Molecular Biology of the Cell 2006 Dec; 26(23):8803.
Application：IF, Monkey, COS-7 cells.
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Polysome-bound endonuclease PMR1 is targeted to stress granules via stress-specific binding to TIA-1.
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