FOXO4 polyclonal antibody
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More Files
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of FOXO4.
Immunogen
A synthetic peptide corresponding to internal region of human FOXO4.
Sequence
C-EAASQDRMPQDLD
Host
Goat
Theoretical MW (kDa)
53.7
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:64000)
Sandwhich ELISA (0.5-1 ug/mL)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
FOXO4 polyclonal antibody (Cat # PAB7254) (1 ug/mL) staining of K-562 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
Sandwich ELISA
FOXO4 polyclonal antibody (Cat # PAB7254) (0.5 ug/mL) as the reporter with the capture rabbit antibody (2.5 ug/mL). -
Gene Info — FOXO4
Entrez GeneID
4303Protein Accession#
NP_005929.2Gene Name
FOXO4
Gene Alias
AFX, AFX1, MGC120490, MLLT7
Gene Description
forkhead box O4
Omim ID
300033Gene Ontology
HyperlinkGene Summary
7|myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog
Other Designations
OTTHUMP00000023496|myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog); translocated to, 7|myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 7
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Interactome
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Disease
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Publication Reference
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FoxOs are lineage-restricted redundant tumor suppressors and regulate endothelial cell homeostasis.
Paik JH, Kollipara R, Chu G, Ji H, Xiao Y, Ding Z, Miao L, Tothova Z, Horner JW, Carrasco DR, Jiang S, Gilliland DG, Chin L, Wong WH, Castrillon DH, DePinho RA.
Cell 2007 Jan; 128(2):309.
Application:WB-Ti, Mouse, Mouse thymi and thymic lymphoma samples.
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FoxOs are lineage-restricted redundant tumor suppressors and regulate endothelial cell homeostasis.
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