PDIA2 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
PDIA2 polyclonal antibody (Cat # PAB7246) (0.03 ug/mL) staining of human pancreas lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of PDIA2.
Immunogen
A synthetic peptide corresponding to human PDIA2.
Sequence
C-NLETTKKYAPVD
Host
Goat
Theoretical MW (kDa)
58.2
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:64000)
Western Blot (0.01-0.03 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
PDIA2 polyclonal antibody (Cat # PAB7246) (0.03 ug/mL) staining of human pancreas lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PDIA2
Entrez GeneID
64714Protein Accession#
NP_006840.2Gene Name
PDIA2
Gene Alias
PDA2, PDI, PDIP, PDIR
Gene Description
protein disulfide isomerase family A, member 2
Omim ID
608012Gene Ontology
HyperlinkGene Summary
Protein disulfide isomerases (EC 5.3.4.1), such as PDIP, are endoplasmic reticulum (ER) resident proteins that catalyze protein folding and thiol-disulfide interchange reactions (Desilva et al., 1996 [PubMed 8561901]).[supplied by OMIM
Other Designations
Rho GDP dissociation inhibitor gamma|pancreatic protein disulfide isomerase|protein disulfide isomerase, pancreatic|protein disulfide isomerase-associated 2
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Interactome
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Disease
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Publication Reference
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S-nitrosylated protein-disulphide isomerase links protein misfolding to neurodegeneration.
Uehara T, Nakamura T, Yao D, Shi ZQ, Gu Z, Ma Y, Masliah E, Nomura Y, Lipton SA.
Nature 2006 May; 441(7092):513.
Application：IF, IP, WB-Tr, Human, HEK 293, HEK 293T, SH-SY5Y cellls.
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S-nitrosylated protein-disulphide isomerase links protein misfolding to neurodegeneration.
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