KLK2 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of KLK2.
Immunogen
A synthetic peptide corresponding to internal region of human KLK2.
Sequence
C-KHQSLRPDEDSSHD
Host
Goat
Theoretical MW (kDa)
28.7, 24.7
Reactivity
Human
Specificity
This antibody is expected to recognize both reported isoforms (NP_005542.1, NP_001002231.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:16000)
Western Blot (0.1-0.3 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2.5-3.8 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
KLK2 polyclonal antibody (Cat # PAB7226) (0.1 ug/mL) staining of human prostate lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
KLK2 polyclonal antibody (Cat # PAB7226) (3.8 ug/mL) staining of paraffin embedded human prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — KLK2
Entrez GeneID
3817Protein Accession#
NP_005542.1;NP_001002231.1Gene Name
KLK2
Gene Alias
KLK2A2, MGC12201, hK2
Gene Description
kallikrein-related peptidase 2
Omim ID
147960Gene Ontology
HyperlinkGene Summary
prostatic
Other Designations
glandular kallikrein 2|kallikrein 2, prostatic
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Interactome
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Disease
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Publication Reference
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Porous silicon microarray for simultaneous fluorometric immunoassay of the biomarkers prostate-specific antigen and human glandular kallikrein 2.
Lee S, Hosokawa K, Kim S, Jeong OC, Lilja H, Laurell T, Maeda M.
Microchimica Acta 2016 Oct; 183(12):3321.
Application:Array, Human, Human serum.
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Simple and robust antibody microarray-based immunoassay platform for sensitive and selective detection of PSA and hK2 toward accurate diagnosis of prostate cancer.
S W Lee, K Hosokawa, S Kim, T Laurell, M Maeda.
Sensing and Bio-Sensing Research 2015 Mar; 3:105.
Application:Det Ab, Human, Human serum, Recombinant proteins.
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Three new serum markers for prostate cancer detection within a percent free PSA-based artificial neural network.
Stephan C, Xu C, Brown DA, Breit SN, Michael A, Nakamura T, Diamandis EP, Meyer H, Cammann H, Jung K.
Prostate 2006 May; 66(6):651.
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Porous silicon microarray for simultaneous fluorometric immunoassay of the biomarkers prostate-specific antigen and human glandular kallikrein 2.
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