pnpla2 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of pnpla2.
Immunogen
A synthetic peptide corresponding to mouse pnpla2.
Sequence
C-NIIEVSKEARKR
Host
Goat
Theoretical MW (kDa)
55.3
Reactivity
Human
Specificity
This antibody was designed using the Mouse sequence NP_080078.1, which differs by 2 amino acids (from the human sequence NP_065109.1.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western blot (0.5-2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
pnpla2 polyclonal antibody (Cat # PAB7224, 0.5 ug/mL) staining of human adipose lysate (35 ug protein in RIPA buffer) . Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
pnpla2 polyclonal antibody (Cat # PAB7224, 2.5 ug/mL) staining of paraffin embedded human heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — Pnpla2
Entrez GeneID
66853Protein Accession#
NP_080078.1Gene Name
Pnpla2
Gene Alias
0610039C21Rik, 1110001C14Rik, Atgl, TTS-2.2
Gene Description
patatin-like phospholipase domain containing 2
Gene Ontology
HyperlinkOther Designations
adipose triglyceride lipase|desnutrin|transport-secretion protein|triglyceride hydrolase
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Publication Reference
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The ATGL gene is associated with free fatty acids, triglycerides, and type 2 diabetes.
Schoenborn V, Heid IM, Vollmert C, Lingenhel A, Adams TD, Hopkins PN, Illig T, Zimmermann R, Zechner R, Hunt SC, Kronenberg F.
Diabetes 2006 May; 55(5):1270.
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The ATGL gene is associated with free fatty acids, triglycerides, and type 2 diabetes.
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