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DGAT2 polyclonal antibody

  • Catalog # : PAB7220
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of DGAT2.
  • Immunogen:
  • A synthetic peptide corresponding to near C terminus of human DGAT2.
  • Sequence:
  • C-KLEHPTQQDIDLYH
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 43.8
  • Reactivity:
  • Human
  • Specificity:
  • This antibody is not expected to cross-react with other DGAT members.
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Recommend Usage:
  • ELISA (1:128000)
    Sandwhich ELISA (0.5-1 ug/mL)
    Western blot (0.3-1 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • DGAT2 polyclonal antibody (Cat # PAB7220, 0.5 ug/mL) staining of human liver lysate in the third lane (35 ug protein in RIPA buffer) . Primary incubation was 1 hour. Detected by chemiluminescence.
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • HEK293 overexpressing DGAT2 (lane 1) and probed with DGAT2 polyclonal antibody (Cat # PAB7220) (mock transfection in lane 2) .
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • DGAT2 polyclonal antibody (Cat # PAB7220, 3 ug/mL) staining of paraffin embedded human liver. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Gene Name:
  • DGAT2
  • Gene Alias:
  • DKFZp686A15125,HMFN1045
  • Gene Description:
  • diacylglycerol O-acyltransferase homolog 2 (mouse)
  • Gene Summary:
  • Acyl-CoA:diacylglycerol acyltransferase, or DGAT (EC 2.3.1.20), is responsible for the synthesis of triglycerides. It catalyzes a reaction in which diacylglycerol is covalently joined to long chain fatty acyl-CoAs.[supplied by OMIM
  • Other Designations:
  • GS1999full,diacylglycerol O-acyltransferase homolog 2,diacylglycerol O-acyltransferase-like protein 2
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