DGAT2 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of DGAT2.
Immunogen
A synthetic peptide corresponding to near C terminus of human DGAT2.
Sequence
C-KLEHPTQQDIDLYH
Host
Goat
Theoretical MW (kDa)
43.8
Reactivity
Human
Specificity
This antibody is not expected to cross-react with other DGAT members.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:128000)
Sandwhich ELISA (0.5-1 ug/mL)
Western blot (0.3-1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
DGAT2 polyclonal antibody (Cat # PAB7220, 0.5 ug/mL) staining of human liver lysate in the third lane (35 ug protein in RIPA buffer) . Primary incubation was 1 hour. Detected by chemiluminescence.Western Blot (Transfected lysate)
HEK293 overexpressing DGAT2 (lane 1) and probed with DGAT2 polyclonal antibody (Cat # PAB7220) (mock transfection in lane 2) .Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
DGAT2 polyclonal antibody (Cat # PAB7220, 3 ug/mL) staining of paraffin embedded human liver. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — DGAT2
Entrez GeneID
84649Protein Accession#
NP_115953.2Gene Name
DGAT2
Gene Alias
DKFZp686A15125, HMFN1045
Gene Description
diacylglycerol O-acyltransferase homolog 2 (mouse)
Omim ID
606983Gene Ontology
HyperlinkGene Summary
Acyl-CoA:diacylglycerol acyltransferase, or DGAT (EC 2.3.1.20), is responsible for the synthesis of triglycerides. It catalyzes a reaction in which diacylglycerol is covalently joined to long chain fatty acyl-CoAs.[supplied by OMIM
Other Designations
GS1999full|diacylglycerol O-acyltransferase homolog 2|diacylglycerol O-acyltransferase-like protein 2
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Interactome
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Disease
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