Actl7b polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of Actl7b.
Immunogen
A synthetic peptide corresponding to mouse Actl7b.
Sequence
C-DELHVDYELPDGK
Host
Goat
Theoretical MW (kDa)
45.6
Reactivity
Rat
Specificity
This antibody is not expected to cross-react with Actin-like 7A.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western Blot (0.5-1.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
The Actl7b polyclonal antibody (Cat # PAB7216) (0.5 ug/mL) staining of rat testis lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — Actl7b
Entrez GeneID
11471Protein Accession#
NP_079547.1Gene Name
Actl7b
Gene Alias
MGC151192, MGC151194, Tact1
Gene Description
actin-like 7b
Gene Ontology
HyperlinkOther Designations
OTTMUSP00000007742|t-actin 1
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Publication Reference
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Cloning, mapping, and expression of two novel actin genes, actin-like-7A (ACTL7A) and actin-like-7B (ACTL7B), from the familial dysautonomia candidate region on 9q31.
Chadwick BP, Mull J, Helbling LA, Gill S, Leyne M, Robbins CM, Pinkett HW, Makalowska I, Maayan C, Blumenfeld A, Axelrod FB, Brownstein M, Gusella JF, Slaugenhaupt SA.
Genomics 1999 Jun; 58(3):302.
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Cloning, mapping, and expression of two novel actin genes, actin-like-7A (ACTL7A) and actin-like-7B (ACTL7B), from the familial dysautonomia candidate region on 9q31.
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