OGT polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
OGT polyclonal antibody (Cat # PAB7209) (0.05 ug/mL) staining of rat pancreas lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of OGT.
Immunogen
A synthetic peptide corresponding to human OGT.
Sequence
C-YEHPKDLKLSDGR
Host
Goat
Theoretical MW (kDa)
117, 116
Reactivity
Mouse, Rat
Specificity
This antibody is expected to recognize both reported isoforms (NP_858058.1 and NP_858059.1.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:64000)
Western Blot (0.05-0.2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
OGT polyclonal antibody (Cat # PAB7209) (0.05 ug/mL) staining of rat pancreas lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — OGT
Entrez GeneID
8473Protein Accession#
NP_858058.1;NP_858059.1Gene Name
OGT
Gene Alias
FLJ23071, HRNT1, MGC22921, O-GLCNAC
Gene Description
O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase)
Omim ID
300255Gene Ontology
HyperlinkGene Summary
O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains nine tetratricopeptide repeats and a putative bipartite nuclear localization signal. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq
Other Designations
O-GlcNAc transferase p110 subunit|O-linked GlcNAc transferase|OTTHUMP00000032154|OTTHUMP00000032166|uridinediphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyl transferase
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Interactome
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Pathway
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Disease
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Publication Reference
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Insulin stimulates and diabetes inhibits O-linked N-acetylglucosamine transferase and O-glycosylation of Sp1.
Majumdar G, Wright J, Markowitz P, Martinez-Hernandez A, Raghow R, Solomon SS.
Diabetes 2004 Dec; 53(12):3184.
Application：IHC, WB-Ce, Rat, H411E cells.
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Insulin stimulates and diabetes inhibits O-linked N-acetylglucosamine transferase and O-glycosylation of Sp1.
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