FAIM polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of FAIM.
Immunogen
A synthetic peptide corresponding to human FAIM.
Sequence
C-KKYMEDRSKTTN
Host
Goat
Theoretical MW (kDa)
24, 22.6, 20.2
Reactivity
Human
Specificity
This antibody is expected to recognize all reported isoforms (NP_001028202.1, NP_00102820.3.1, NP_001028204.1, NP_060617.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western blot (0.5-1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
FAIM polyclonal antibody (Cat # PAB7205) (0.5 ug/mL) staining of human lymph node lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
FAIM polyclonal antibody (Cat # PAB7205, 2.5 ug/mL) staining of paraffin embedded human placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — FAIM
Entrez GeneID
55179Protein Accession#
NP_001028202.1;NP_00102820.3.1;NP_001028204.1;NP_060617.1Gene Name
FAIM
Gene Alias
FAIM1
Gene Description
Fas apoptotic inhibitory molecule
Gene Ontology
HyperlinkOther Designations
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Interactome
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Disease
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Publication Reference
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A novel gene coding for a Fas apoptosis inhibitory molecule (FAIM) isolated from inducibly Fas-resistant B lymphocytes.
Schneider TJ, Fischer GM, Donohoe TJ, Colarusso TP, Rothstein TL.
The Journal of Experimental Medicine 1999 Mar; 189(6):949.
Application:WB-Tr, Mouse, B cells, BAL-17 cells.
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A novel gene coding for a Fas apoptosis inhibitory molecule (FAIM) isolated from inducibly Fas-resistant B lymphocytes.
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