ADH5 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of ADH5.
Immunogen
A synthetic peptide corresponding to internal region of human ADH5.
Sequence
C-KKIKVDEFVTHN
Host
Goat
Theoretical MW (kDa)
39.7
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:4000)
Western Blot (0.5-1.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
ADH5 polyclonal antibody (Cat # PAB7204) (0.5 ug/mL) staining of human testis lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ADH5
Entrez GeneID
128Protein Accession#
NP_000662.3Gene Name
ADH5
Gene Alias
ADH-3, ADHX, FDH, GSNOR
Gene Description
alcohol dehydrogenase 5 (class III), chi polypeptide
Omim ID
103710Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. The encoded protein forms a homodimer. It has virtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long-chain primary alcohols and for oxidation of S-hydroxymethyl-glutathione, a spontaneous adduct between formaldehyde and glutathione. This enzyme is an important component of cellular metabolism for the elimination of formaldehyde, a potent irritant and sensitizing agent that causes lacrymation, rhinitis, pharyngitis, and contact dermatitis. The human genome contains several non-transcribed pseudogenes related to this gene. [provided by RefSeq
Other Designations
alcohol dehydrogenase (class III), chi polypeptide|class III alcohol dehydrogenase 5|formaldehyde dehydrogenase|glutathione-dependent formaldehyde dehydrogenase
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Interactome
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Pathway
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Disease
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Publication Reference
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Immunological and biochemical characterization of the human alcohol dehydrogenase chi-ADH isozyme.
Adinolfi A, Adinolfi M, Hopkinson DA.
Annals of Human Genetics 1984 Jan; 48(1):1.
Application:SDS-PAGE, WB-Re, Human, Human liver extracts, Purified proteins.
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Immunological and biochemical characterization of the human alcohol dehydrogenase chi-ADH isozyme.
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