NCDN polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot (Cell lysate) analysis of (1) HepG2, (2) U251 and (3) peptide (35 ug protein in RIPA buffer).
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of NCDN.
Immunogen
A synthetic peptide corresponding to human NCDN.
Sequence
C-HYLLQVGSEKQKEP
Host
Goat
Theoretical MW (kDa)
78.9, 77.2
Specificity
This antibody is expected to recognize isoform 1 (NP_055099.1; NP_001014839.1) and isoform 2 (NP_001014841.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:16000)
Western Blot (0.3-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot (Cell lysate) analysis of (1) HepG2, (2) U251 and (3) peptide (35 ug protein in RIPA buffer).Enzyme-linked Immunoabsorbent Assay
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Gene Info — NCDN
Entrez GeneID
23154Protein Accession#
NP_055099.1;NP_001014839.1;NP_001014841.1Gene Name
NCDN
Gene Alias
KIAA0607
Gene Description
neurochondrin
Omim ID
608458Gene Ontology
HyperlinkGene Summary
This gene encodes a leucine-rich cytoplasmic protein, which is highly similar to a mouse protein that negatively regulates Ca/calmodulin-dependent protein kinase II phosphorylation and may be essential for spatial learning processes. Several alternatively spliced transcript variants of this gene have been described. [provided by RefSeq
Other Designations
OTTHUMP00000065369|OTTHUMP00000065370|OTTHUMP00000065371
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Interactome
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Publication Reference
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Neurochondrin negatively regulates CaMKII phosphorylation, and nervous system-specific gene disruption results in epileptic seizure.
Dateki M, Horii T, Kasuya Y, Mochizuki R, Nagao Y, Ishida J, Sugiyama F, Tanimoto K, Yagami K, Imai H, Fukamizu A.
The Journal of Biological Chemistry 2005 May; 280(21):20503.
Application：WB-Ti, WB-Tr, Mouse, Brain, NIH3T3 cells.
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Neurochondrin negatively regulates CaMKII phosphorylation, and nervous system-specific gene disruption results in epileptic seizure.
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