TBC1D4 polyclonal antibody
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More Files
- More Functions
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of TBC1D4.
Immunogen
A synthetic peptide corresponding to human TBC1D4.
Sequence
C-DDPEKIEERKKSK
Host
Goat
Theoretical MW (kDa)
147
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:128000)
Western blot (0.1-0.3 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
TBC1D4 polyclonal antibody (Cat # PAB7192) (0.1 ug/mL) staining of Daudi cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
TBC1D4 polyclonal antibody (Cat # PAB7192, 3.8 ug/mL) staining of paraffin embedded human skeletal muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — TBC1D4
Entrez GeneID
9882Protein Accession#
NP_055647.2Gene Name
TBC1D4
Gene Alias
AS160, DKFZp779C0666
Gene Description
TBC1 domain family, member 4
Gene Ontology
HyperlinkGene Summary
O
Other Designations
Acrg embryonic lethality minimal region ortholog|OTTHUMP00000018505|OTTHUMP00000040818|TBC (Tre-2, BUB2, CDC16) domain-containing protein
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Interactome
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Disease
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Publication Reference
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Insulin-stimulated phosphorylation of the Akt substrate AS160 is impaired in skeletal muscle of type 2 diabetic subjects.
Karlsson HK, Zierath JR, Kane S, Krook A, Lienhard GE, Wallberg-Henriksson H.
Diabetes 2005 Jun; 54(6):1692.
Application:IP, WB-Ti, Human, Muscle.
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Insulin-stimulated phosphorylation of the Akt substrate AS160 is impaired in skeletal muscle of type 2 diabetic subjects.
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