TCF3 polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Cell lysate)
TCF3 polyclonal antibody (Cat # PAB7188) (0.01 ug/mL) staining of Daudi cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
TCF3 polyclonal antibody (Cat # PAB7188) (3.8 ug/mL) staining of paraffin embedded human kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
-
Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of TCF3.
Immunogen
A synthetic peptide corresponding to human TCF3.
Sequence
C-KAPRARTSPDEDED
Host
Goat
Theoretical MW (kDa)
67.5
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:128000)
Western blot (0.01-0.03 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
TCF3 polyclonal antibody (Cat # PAB7188) (0.01 ug/mL) staining of Daudi cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
TCF3 polyclonal antibody (Cat # PAB7188) (3.8 ug/mL) staining of paraffin embedded human kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
-
Gene Info — TCF3
Entrez GeneID
6929Protein Accession#
NP_003191.1Gene Name
TCF3
Gene Alias
E2A, ITF1, MGC129647, MGC129648, bHLHb21
Gene Description
transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47)
Omim ID
147141Gene Ontology
HyperlinkGene Summary
The TCF3 gene, also called E2A, encodes 2 basic helix-loop-helix (bHLH) transcription factors, E12 and E47, through alternative splicing. E12 and E47 are involved in regulation of immunoglobulin gene expression (Bain et al., 1994 [PubMed 8001125]).[supplied by OMIM
Other Designations
E2A immunoglobulin enhancer-binding factor E12/E47|immunoglobulin transcription factor 1|kappa-E2-binding factor|transcription factor 3|transcription factor E2-alpha
-
Interactome
-
Publication Reference
-
Differential functions for the transcription factor E2A in positive and negative gene regulation in pre-B lymphocytes.
Greenbaum S, Lazorchak AS, Zhuang Y.
The Journal of Biological Chemistry 2004 Oct; 279(43):45028.
Application：Func, Mouse , Mouse pre-B cells.
-
Differential functions for the transcription factor E2A in positive and negative gene regulation in pre-B lymphocytes.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com