DGAT1 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
DGAT1 polyclonal antibody (Cat # PAB7180) (0.5 ug/mL) staining of human duodenum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of DGAT1.
Immunogen
A synthetic peptide corresponding to human DGAT1.
Sequence
C-QNSMKPFKDMDYS
Host
Goat
Theoretical MW (kDa)
55.3
Reactivity
Human, Rat
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:16000)
Western Blot (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
DGAT1 polyclonal antibody (Cat # PAB7180) (0.5 ug/mL) staining of human duodenum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — DGAT1
Entrez GeneID
8694Protein Accession#
NP_0.36211.1Gene Name
DGAT1
Gene Alias
ARGP1, DGAT
Gene Description
diacylglycerol O-acyltransferase homolog 1 (mouse)
Omim ID
604900Gene Ontology
HyperlinkGene Summary
The enzyme encoded by this gene utilizes diacylglycerol and fatty acyl CoA as substrates in order to catalyze the final stage of triacylglycerol synthesis. It is also involved in cellular as well as physiological metabolic processes. [provided by RefSeq
Other Designations
ACAT related gene product 1|acyl coenzyme A:cholesterol acyltransferase related gene 1|diacylglycerol O-acyltransferase 1|diglyceride acyltransferase
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Interactome
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Pathway
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Disease
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Publication Reference
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Overexpression of diacylglycerol acyltransferase-1 reduces phospholipid synthesis, proliferation, and invasiveness in simian virus 40-transformed human lung fibroblasts.
Bagnato C, Igal RA.
The Journal of Biological Chemistry 2003 Dec; 278(52):52203.
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Overexpression of diacylglycerol acyltransferase-1 reduces phospholipid synthesis, proliferation, and invasiveness in simian virus 40-transformed human lung fibroblasts.
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