ARF1/ARF2/ARF3/ARF4 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
The ARF1/ARF2/ARF3/ARF4 polyclonal antibody (Cat # PAB7171) (1 ug/mL) staining of A431 (1) and (0.3 ug/mL) of HeLa (2) and MCF7 (3) NIH3T3 (4), KNRK (5) and MDCK (6) cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Flow Cytometry
Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of ARF1/ARF2/ARF3/ARF4.
Immunogen
A synthetic peptide corresponding to human ARF1/ARF2/ARF3/ARF4.
Sequence
C-EGLDWLSNQLRNQK
Host
Goat
Theoretical MW (kDa)
20.7
Reactivity
Dog, Human, Mouse, Rat
Specificity
This antibody is expected to recognize all four reported isoforms.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:8000)
Flow Cytometry (10 ug/mL)
Western Blot (0.3-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
The ARF1/ARF2/ARF3/ARF4 polyclonal antibody (Cat # PAB7171) (1 ug/mL) staining of A431 (1) and (0.3 ug/mL) of HeLa (2) and MCF7 (3) NIH3T3 (4), KNRK (5) and MDCK (6) cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody. -
Gene Info — ARF1
Entrez GeneID
375Protein Accession#
NP_001019398.1 (Gene ID : 375);NP_001019397.1 (Gene ID : 375);NP_001019399.1 (Gene ID : 375);NP_001649.1 (Gene ID : 375);NP_001649.1 (Gene ID : 375)Gene Name
ARF1
Gene Alias
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Gene Description
ADP-ribosylation factor 1
Omim ID
103180Gene Ontology
HyperlinkGene Summary
ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000035715
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Gene Info — ARF3
Entrez GeneID
377Protein Accession#
NP_001019398.1 (Gene ID : 375);NP_001019397.1 (Gene ID : 375);NP_001019399.1 (Gene ID : 375);NP_001649.1 (Gene ID : 375);NP_001649.1 (Gene ID : 375)Gene Name
ARF3
Gene Alias
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Gene Description
ADP-ribosylation factor 3
Omim ID
103190Gene Ontology
HyperlinkGene Summary
ADP-ribosylation factor 3 (ARF3) is a member of the human ARF gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6) and members of each class share a common gene organization. The ARF3 gene contains five exons and four introns. [provided by RefSeq
Other Designations
ADP-ribosylation factor protein 3|small GTP binding protein
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Gene Info — ARF4
Entrez GeneID
378Protein Accession#
NP_001019398.1 (Gene ID : 375);NP_001019397.1 (Gene ID : 375);NP_001019399.1 (Gene ID : 375);NP_001649.1 (Gene ID : 375);NP_001649.1 (Gene ID : 375)Gene Name
ARF4
Gene Alias
ARF2
Gene Description
ADP-ribosylation factor 4
Omim ID
601177Gene Ontology
HyperlinkGene Summary
This gene is a member of the human ARF gene family whose members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 5 ARF proteins and 11 ARF-like proteins and constitute one family of the RAS superfamily. The ARF proteins are categorized as class I, class II and class III; this gene is a class II member. The members of each class share a common gene organization. The ARF4 gene spans approximately 12kb and contains six exons and five introns. This gene is the most divergent member of the human ARFs. Conflicting map positions at 3p14 or 3p21 have been reported for this gene. [provided by RefSeq
Other Designations
ADP-ribosylation factor 2
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Interactome
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Pathway
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Disease
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Publication Reference
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Golgi-localized GAP for Cdc42 functions downstream of ARF1 to control Arp2/3 complex and F-actin dynamics.
Dubois T, Paleotti O, Mironov AA, Fraisier V, Stradal TE, De Matteis MA, Franco M, Chavrier P.
Nature Cell Biology 2005 Apr; 7(4):353.
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Golgi-localized GAP for Cdc42 functions downstream of ARF1 to control Arp2/3 complex and F-actin dynamics.
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