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Last updated: 2016/12/4
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ARF1/ARF2/ARF3/ARF4 polyclonal antibody

  • Catalog # : PAB7171
  • Visit Frequency :
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of ARF1/ARF2/ARF3/ARF4.
  • Immunogen:
  • A synthetic peptide corresponding to human ARF1/ARF2/ARF3/ARF4.
  • Sequence:
  • C-EGLDWLSNQLRNQK
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 20.7
  • Reactivity:
  • Human
  • Specificity:
  • This antibody is expected to recognize all four reported isoforms.
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:8000)
    Western Blot (1-3 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • The ARF1/ARF2/ARF3/ARF4 polyclonal antibody (Cat # PAB7171) (1 ug/mL) staining of HepG2 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 375
  • Protein Accession#:
  • NP_001019398.1 (Gene ID : 375);NP_001019397.1 (Gene ID : 375);NP_001019399.1 (Gene ID : 375);NP_001649.1 (Gene ID : 375);NP_001649.1 (Gene ID : 375)
  • Gene Name:
  • ARF1
  • Gene Alias:
  • -
  • Gene Description:
  • ADP-ribosylation factor 1
  • Gene Summary:
  • ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000035715
  • Gene Information
  • Entrez GeneID:
  • 377
  • Protein Accession#:
  • NP_001019398.1 (Gene ID : 375);NP_001019397.1 (Gene ID : 375);NP_001019399.1 (Gene ID : 375);NP_001649.1 (Gene ID : 375);NP_001649.1 (Gene ID : 375)
  • Gene Name:
  • ARF3
  • Gene Alias:
  • -
  • Gene Description:
  • ADP-ribosylation factor 3
  • Gene Summary:
  • ADP-ribosylation factor 3 (ARF3) is a member of the human ARF gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6) and members of each class share a common gene organization. The ARF3 gene contains five exons and four introns. [provided by RefSeq
  • Other Designations:
  • ADP-ribosylation factor protein 3,small GTP binding protein
  • Gene Information
  • Entrez GeneID:
  • 378
  • Protein Accession#:
  • NP_001019398.1 (Gene ID : 375);NP_001019397.1 (Gene ID : 375);NP_001019399.1 (Gene ID : 375);NP_001649.1 (Gene ID : 375);NP_001649.1 (Gene ID : 375)
  • Gene Name:
  • ARF4
  • Gene Alias:
  • ARF2
  • Gene Description:
  • ADP-ribosylation factor 4
  • Gene Summary:
  • This gene is a member of the human ARF gene family whose members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 5 ARF proteins and 11 ARF-like proteins and constitute one family of the RAS superfamily. The ARF proteins are categorized as class I, class II and class III; this gene is a class II member. The members of each class share a common gene organization. The ARF4 gene spans approximately 12kb and contains six exons and five introns. This gene is the most divergent member of the human ARFs. Conflicting map positions at 3p14 or 3p21 have been reported for this gene. [provided by RefSeq
  • Other Designations:
  • ADP-ribosylation factor 2
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