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Last updated: 2016/11/27
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MBD2 polyclonal antibody

  • Catalog # : PAB7163
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of MBD2.
  • Immunogen:
  • A synthetic peptide corresponding to internal region of human MBD2.
  • Sequence:
  • C-RNDPLNQNKGKPDLN
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 43.3
  • Reactivity:
  • Human
  • Specificity:
  • This antibody is expected to recognize isoform 1 (NP_0.03918.1) only.
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Recommend Usage:
  • ELISA (1:64000)
    Western blot (0.3-1 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2-4 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • MBD2 polyclonal antibody (Cat # PAB7163) (0.3 ug/mL) staining of Jurkat cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • MBD2 polyclonal antibody (Cat # PAB7163) (2.5 ug/mL) staining of paraffin embedded human colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 8932
  • Gene Name:
  • MBD2
  • Gene Alias:
  • DKFZp586O0821,DMTase,NY-CO-41
  • Gene Description:
  • methyl-CpG binding domain protein 2
  • Gene Summary:
  • DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing. [provided by RefSeq
  • Other Designations:
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