MBD2 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
MBD2 polyclonal antibody (Cat # PAB7163) (0.3 ug/mL) staining of Jurkat cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
MBD2 polyclonal antibody (Cat # PAB7163) (2.5 ug/mL) staining of paraffin embedded human colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of MBD2.
Immunogen
A synthetic peptide corresponding to internal region of human MBD2.
Sequence
C-RNDPLNQNKGKPDLN
Host
Goat
Theoretical MW (kDa)
43.3
Reactivity
Human
Specificity
This antibody is expected to recognize isoform 1 (NP_0.03918.1) only.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:64000)
Western blot (0.3-1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
MBD2 polyclonal antibody (Cat # PAB7163) (0.3 ug/mL) staining of Jurkat cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
MBD2 polyclonal antibody (Cat # PAB7163) (2.5 ug/mL) staining of paraffin embedded human colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — MBD2
Entrez GeneID
8932Protein Accession#
NP_0.03918.1Gene Name
MBD2
Gene Alias
DKFZp586O0821, DMTase, NY-CO-41
Gene Description
methyl-CpG binding domain protein 2
Omim ID
603547Gene Ontology
HyperlinkGene Summary
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing. [provided by RefSeq
Other Designations
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Interactome
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Disease
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Publication Reference
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Specific binding of the methyl binding domain protein 2 at the BRCA1-NBR2 locus.
Auriol E, Billard LM, Magdinier F, Dante R.
Nucleic Acids Research 2005 Jul; 33(13):4243.
Application：ChIP, WB-Tr, Human, HeLa cells.
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Specific binding of the methyl binding domain protein 2 at the BRCA1-NBR2 locus.
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