CCM2 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of CCM2.
Immunogen
A synthetic peptide corresponding to human CCM2.
Sequence
C-KGEKSRDKKAHEK
Host
Goat
Theoretical MW (kDa)
51.4, 48.8
Reactivity
Human
Specificity
This antibody is expected to recognize both reported isoforms (NP_001025006.1 and NP_113631.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:128000)
Western Blot (0.03-0.1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
CCM2 polyclonal antibody (Cat # PAB7146) (0.03 ug/mL) staining of human heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — CCM2
Entrez GeneID
83605Protein Accession#
NP_001025006.1;NP_113631.1Gene Name
CCM2
Gene Alias
C7orf22, MGC4067, MGC4607, MGC74868, PP10187
Gene Description
cerebral cavernous malformation 2
Gene Ontology
HyperlinkOther Designations
malcavernin
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Interactome
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Disease
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Publication Reference
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CCM1 and CCM2 protein interactions in cell signaling: implications for cerebral cavernous malformations pathogenesis.
Zawistowski JS, Stalheim L, Uhlik MT, Abell AN, Ancrile BB, Johnson GL, Marchuk DA.
Human Molecular Genetics 2005 Sep; 14(17):2521.
Application:IP, WB-Tr, Human, Mouse, HEK 293T cells, MEFs.
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CCM1 and CCM2 protein interactions in cell signaling: implications for cerebral cavernous malformations pathogenesis.
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