S100A4 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
S100A4 polyclonal antibody (Cat # PAB7142) (0.03 ug/mL) staining of human placenta lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
S100A4 polyclonal antibody (Cat # PAB7142, 2.5 ug/mL) staining of paraffin embedded human small intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of S100A4.
Immunogen
A synthetic peptide corresponding to human S100A4.
Sequence
CNEFFEGFPDKQP
Host
Goat
Theoretical MW (kDa)
11.7
Reactivity
Human
Specificity
Both variants (NP_002952.1; NP_062427.1) represent identical protein.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:128000)
Western blot (0.03-0.1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
S100A4 polyclonal antibody (Cat # PAB7142) (0.03 ug/mL) staining of human placenta lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
S100A4 polyclonal antibody (Cat # PAB7142, 2.5 ug/mL) staining of paraffin embedded human small intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — S100A4
Entrez GeneID
6275Protein Accession#
NP_002952.1;NP_062427.1Gene Name
S100A4
Gene Alias
18A2, 42A, CAPL, FSP1, MTS1, P9KA, PEL98
Gene Description
S100 calcium binding protein A4
Omim ID
114210Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq
Other Designations
OTTHUMP00000015467|OTTHUMP00000015468|OTTHUMP00000015469|OTTHUMP00000032895|S100 calcium binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog)|S100 calcium-binding protein A4|S100 calcium-binding protein A4 (calcium prote
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Interactome
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Disease
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Publication Reference
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Terminal fucose mediates progression of human cholangiocarcinoma through EGF/EGFR activation and the Akt/Erk signaling pathway.
Indramanee S, Sawanyawisuth K, Silsirivanit A, Dana P, Phoomak C, Kariya R, Klinhom-On N, Sorin S, Wongkham C, Okada S, Wongkham S.
Scientific Reports 2019 Nov; 9(1):17266.
Application：WB-Tr, Human, Human cholangiocarcinoma cells.
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The S100A4 metastasis factor regulates cellular motility via a direct interaction with myosin-IIA.
Li ZH, Bresnick AR.
Cancer Research 2006 May; 66(10):5173.
Application：IF, WB-Tr, Mouse, MTC cells.
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Terminal fucose mediates progression of human cholangiocarcinoma through EGF/EGFR activation and the Akt/Erk signaling pathway.
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