GFAP polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of GFAP.
Immunogen
A synthetic peptide corresponding to C-terminus of human GFAP.
Sequence
C-DGEVIKESKQEHKD
Host
Goat
Theoretical MW (kDa)
49.9
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Western Blot (0.001-0.01 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
GFAP polyclonal antibody (Cat # PAB7137) (0.01 ug/mL) staining of Human Cerebellum (1), Mouse Brain (2) and (0.003 ug/mL) Rat Brain (3) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence. -
Gene Info — GFAP
Entrez GeneID
2670Protein Accession#
NP_002046.1Gene Name
GFAP
Gene Alias
FLJ45472
Gene Description
glial fibrillary acidic protein
Gene Ontology
HyperlinkGene Summary
This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq
Other Designations
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Interactome
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Disease
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Publication Reference
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Proteomic analysis of glial fibrillary acidic protein in Alzheimer's disease and aging brain.
Korolainen MA, Auriola S, Nyman TA, Alafuzoff I, Pirttila T.
Neurobiology of Disease 2005 Dec; 20(3):858.
Application:2D, WB-Ti, Human, Brain.
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Proteomic analysis of glial fibrillary acidic protein in Alzheimer's disease and aging brain.
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