PEBP1 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
PEBP1 polyclonal antibody (Cat # PAB7136) (0.01 ug/mL) staining of human prostate lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
PEBP1 polyclonal antibody (Cat # PAB7136) (2.5 ug/mL) staining of paraffin embedded human adrenal gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of PEBP1.
Immunogen
A synthetic peptide corresponding to human PEBP1.
Sequence
C-DPDAPSRKDPKYRE
Host
Goat
Theoretical MW (kDa)
21.1
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:128000)
Western blot (0.01-0.03 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
PEBP1 polyclonal antibody (Cat # PAB7136) (0.01 ug/mL) staining of human prostate lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
PEBP1 polyclonal antibody (Cat # PAB7136) (2.5 ug/mL) staining of paraffin embedded human adrenal gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PEBP1
Entrez GeneID
5037Protein Accession#
NP_002558.1Gene Name
PEBP1
Gene Alias
HCNP, PBP, PEBP, RKIP
Gene Description
phosphatidylethanolamine binding protein 1
Omim ID
604591Gene Ontology
HyperlinkOther Designations
Raf kinase inhibitory protein|prostatic binding protein
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Interactome
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Publication Reference
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Suppression of Raf-1 kinase activity and MAP kinase signalling by RKIP.
Yeung K, Seitz T, Li S, Janosch P, McFerran B, Kaiser C, Fee F, Katsanakis KD, Rose DW, Mischak H, Sedivy JM, Kolch W.
Nature 1999 Sep; 401(6749):173.
Application：IF, IP-WB, WB-Ce, WB-Tr, Mouse, Rat, Rat-1, NIH/3T3 cells, 208F fibroblasts.
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Suppression of Raf-1 kinase activity and MAP kinase signalling by RKIP.
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