TCFL5 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of TCFL5.
Immunogen
A synthetic peptide corresponding to human TCFL5.
Sequence
C-KYIQERHGDSLKKE
Host
Goat
Theoretical MW (kDa)
52.7
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:64000)
Western Blot (0.3-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
TCFL5 polyclonal antibody (Cat # PAB7123) (0.3 ug/mL) staining of human testis lysate (35 ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — TCFL5
Entrez GeneID
10732Protein Accession#
NP_006593.2Gene Name
TCFL5
Gene Alias
CHA, E2BP-1, Figlb, MGC46135
Gene Description
transcription factor-like 5 (basic helix-loop-helix)
Omim ID
604745Gene Ontology
HyperlinkOther Designations
HPV-16 E2 binding protein 1|transcription factor-like 5 protein
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Interactome
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Publication Reference
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Basic helix-loop-helix transcription factor Tcfl5 interacts with the Calmegin gene promoter in mouse spermatogenesis.
Siep M, Sleddens-Linkels E, Mulders S, van Eenennaam H, Wassenaar E, Van Cappellen WA, Hoogerbrugge J, Grootegoed JA, Baarends WM.
Nucleic Acids Research 2004 Dec; 32(21):6425.
Application:IF, IHC-P, WB, Mouse , Mouse spermatocytes, testis.
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Basic helix-loop-helix transcription factor Tcfl5 interacts with the Calmegin gene promoter in mouse spermatogenesis.
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