NHEJ1 polyclonal antibody
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More Files
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of NHEJ1.
Immunogen
A synthetic peptide corresponding to C terminus of human NHEJ1.
Sequence
C-QRPQLSKVKRKKPR
Host
Goat
Theoretical MW (kDa)
33.3
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:64000)
Western Blot (0.1-0.3 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2.5-3.8 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
NHEJ1 polyclonal antibody (Cat # PAB7119) (0.1 ug/mL) staining of human thyroid lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
NHEJ1 polyclonal antibody (Cat # PAB7119) (3.8 ug/mL) staining of paraffin embedded human placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — NHEJ1
Entrez GeneID
79840Protein Accession#
NP_079058.1Gene Name
NHEJ1
Gene Alias
FLJ12610, XLF
Gene Description
nonhomologous end-joining factor 1
Omim ID
611290Gene Ontology
HyperlinkGene Summary
Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders. [provided by RefSeq
Other Designations
Cernunnos|XRCC4-like factor
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Interactome
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Pathway
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Disease
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Publication Reference
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XLF interacts with the XRCC4-DNA ligase IV complex to promote DNA nonhomologous end-joining.
Ahnesorg P, Smith P, Jackson SP.
Cell 2006 Jan; 124(2):301.
Application:IF, IP-WB, WB-Ce, WB-Tr, Human, HeLa, 293T, U2OS, 2BN, MRC5, BJ, 2BN-C237 cells.
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XLF interacts with the XRCC4-DNA ligase IV complex to promote DNA nonhomologous end-joining.
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