SV2A polyclonal antibody
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More Files
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Immunofluorescence
SV2A polyclonal antibody (Cat # PAB7115) Immunofluorescence analysis of paraformaldehyde fixed KNRK cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunofluorescence
SV2A polyclonal antibody (Cat # PAB7115) Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of SV2A.
Immunogen
A synthetic peptide corresponding to human SV2A.
Sequence
C-TVELYPSDKRTTA
Host
Goat
Theoretical MW (kDa)
82.7
Reactivity
Rat
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:1000)
Immunofluorescence (10 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunofluorescence
SV2A polyclonal antibody (Cat # PAB7115) Immunofluorescence analysis of paraformaldehyde fixed KNRK cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).Immunofluorescence
SV2A polyclonal antibody (Cat # PAB7115) Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).Enzyme-linked Immunoabsorbent Assay
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Gene Info — SV2A
Entrez GeneID
9900Protein Accession#
NP_055664.2Gene Name
SV2A
Gene Alias
KIAA0736, SV2
Gene Description
synaptic vesicle glycoprotein 2A
Omim ID
185860Gene Ontology
HyperlinkOther Designations
OTTHUMP00000014065|synaptic vesicle glycoprotein 2
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Interactome
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Pathway
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Disease
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Publication Reference
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SV2 is the protein receptor for botulinum neurotoxin A.
Dong M, Yeh F, Tepp WH, Dean C, Johnson EA, Janz R, Chapman ER.
Science 2006 Apr; 312(5773):592.
Application：IF, IP, WB, Mouse, Rat, Mouse brain, Rat brain.
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SV2 is the protein receptor for botulinum neurotoxin A.
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