CPT1A polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
CPT1A polyclonal antibody (Cat # PAB7110) (0.1 ug/mL) staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
CPT1A polyclonal antibody (Cat # PAB7110) (4 ug/mL) staining of paraffin embedded human kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of CPT1A.
Immunogen
A synthetic peptide corresponding to human CPT1A.
Sequence
C-DPAQTVEQRLKLFK
Host
Goat
Theoretical MW (kDa)
88.4, 86.2
Reactivity
Human
Specificity
This antibody is expected to recognize both reported isoforms (NP_001867.2, NP_001027017.1) but is not expected to recognize CPT1B.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:128000)
Western blot (0.1-0.3 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (4-6 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
CPT1A polyclonal antibody (Cat # PAB7110) (0.1 ug/mL) staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
CPT1A polyclonal antibody (Cat # PAB7110) (4 ug/mL) staining of paraffin embedded human kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — CPT1A
Entrez GeneID
1374Protein Accession#
NP_001867.2;NP_001027017.1Gene Name
CPT1A
Gene Alias
CPT1, CPT1-L, L-CPT1
Gene Description
carnitine palmitoyltransferase 1A (liver)
Gene Ontology
HyperlinkGene Summary
The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
carnitine palmitoyltransferase 1A|carnitine palmitoyltransferase I, liver
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Interactome
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Pathway
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Disease
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Publication Reference
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Restoration of hypothalamic lipid sensing normalizes energy and glucose homeostasis in overfed rats.
Pocai A, Lam TK, Obici S, Gutierrez-Juarez R, Muse ED, Arduini A, Rossetti L.
The Journal of Clinical Investigation 2006 Apr; 116(4):1081.
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Restoration of hypothalamic lipid sensing normalizes energy and glucose homeostasis in overfed rats.
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