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Last updated: 2016/12/4
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CPT1A polyclonal antibody

  • Catalog # : PAB7110
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of CPT1A.
  • Immunogen:
  • A synthetic peptide corresponding to human CPT1A.
  • Sequence:
  • C-DPAQTVEQRLKLFK
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 88.4, 86.2
  • Reactivity:
  • Human
  • Specificity:
  • This antibody is expected to recognize both reported isoforms (NP_001867.2, NP_001027017.1) but is not expected to recognize CPT1B.
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:128000)
    Western blot (0.1-0.3 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (4-6 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • CPT1A polyclonal antibody (Cat # PAB7110) (0.1 ug/mL) staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • CPT1A polyclonal antibody (Cat # PAB7110) (4 ug/mL) staining of paraffin embedded human kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 1374
  • Protein Accession#:
  • NP_001867.2;NP_001027017.1
  • Gene Name:
  • CPT1A
  • Gene Alias:
  • CPT1,CPT1-L,L-CPT1
  • Gene Description:
  • carnitine palmitoyltransferase 1A (liver)
  • Gene Summary:
  • The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • carnitine palmitoyltransferase 1A,carnitine palmitoyltransferase I, liver
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