VHL polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of VHL.
Immunogen
A synthetic peptide corresponding to internal region of human VHL.
Sequence
C-RSLVKPENYRRLD
Host
Goat
Theoretical MW (kDa)
24.2, 19.7
Reactivity
Human, Mouse, Rat
Specificity
This antibody is expected to recognize both reported isoforms (NP_000542.1 and NP_937799.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:128000)
Sandwhich ELISA (1.5-3 ug/mL)
Western Blot (0.01-0.03 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
VHL polyclonal antibody (Cat # PAB7071) (0.01 ug/mL) staining of human ovary lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
Sandwich ELISA
VHL polyclonal antibody (Cat # PAB7071) (1.5 ug/mL) as the reporter as the capture rabbit antibody (2.5 ug/mL). -
Gene Info — VHL
Entrez GeneID
7428Protein Accession#
NP_000542.1;NP_937799.1Gene Name
VHL
Gene Alias
HRCA1, RCA1, VHL1
Gene Description
von Hippel-Lindau tumor suppressor
Gene Ontology
HyperlinkGene Summary
Von Hippel-Lindau syndrome (VHL) is a dominantly inherited familial cancer syndrome predisposing to a variety of malignant and benign tumors. A germline mutation of this gene is the basis of familial inheritance of VHL syndrome. The protein encoded by this gene is a component of the protein complex that includes elongin B, elongin C, and cullin-2, and possesses ubiquitin ligase E3 activity. This protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. RNA polymerase II subunit POLR2G/RPB7 is also reported to be a target of this protein. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq
Other Designations
elongin binding protein
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Interactome
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Pathway
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Disease
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Publication Reference
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Histone deacetylase inhibitors induce VHL and ubiquitin-independent proteasomal degradation of hypoxia-inducible factor 1alpha.
Kong X, Lin Z, Liang D, Fath D, Sang N, Caro J.
Molecular and Cellular Biology 2006 Mar; 26(6):2019.
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Histone deacetylase inhibitors induce VHL and ubiquitin-independent proteasomal degradation of hypoxia-inducible factor 1alpha.
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