WT1 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of WT1.
Immunogen
A synthetic peptide corresponding to human WT1.
Sequence
QDPASTCVPEPASQH
Host
Goat
Theoretical MW (kDa)
54.3, 56, 54.6, 56.3
Reactivity
Human
Specificity
This antibody is expected to recognize all four reported isoforms (NP_000369.3, NP_077742.2, NP_077743.2, NP_077744.3).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:4000)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
WT1 polyclonal antibody (Cat # PAB7070) (1 ug/mL) staining of human spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — WT1
Entrez GeneID
7490Protein Accession#
NP_000369.3;NP_077742.2;NP_077743.2;NP_077744.3Gene Name
WT1
Gene Alias
GUD, WAGR, WIT-2, WT33
Gene Description
Wilms tumor 1
Gene Ontology
HyperlinkGene Summary
This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors. Multiple transcript variants, resulting from alternative splicing at two coding exons, have been well characterized. There is also evidence for the use of non-AUG (CUG) translation initiation site upstream of, and in-frame with the first AUG, leading to additional isoforms. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq
Other Designations
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Interactome
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Disease
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Publication Reference
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Tissue-specific RNAi reveals that WT1 expression in nurse cells controls germ cell survival and spermatogenesis.
Rao MK, Pham J, Imam JS, MacLean JA, Murali D, Furuta Y, Sinha-Hikim AP, Wilkinson MF.
Genes & Development 2006 Jan; 20(2):147.
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Tissue-specific RNAi reveals that WT1 expression in nurse cells controls germ cell survival and spermatogenesis.
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