GPX7 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of GPX7.
Immunogen
A synthetic peptide corresponding to internal region of human GPX7.
Sequence
CNQFGQQEPDSNK
Host
Goat
Theoretical MW (kDa)
21
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Sandwhich ELISA (5-10 ug/mL)
Western Blot (0.3-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
GPX7 polyclonal antibody (Cat # PAB7067) (0.3 ug/mL) staining of human prostate lysate (35 ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
Sandwich ELISA
GPX7 polyclonal antibody (Cat # PAB7067) (5 ug/mL) as the reporter with the capture rabbit antibody (5 ug/mL). -
Gene Info — GPX7
Entrez GeneID
2882Protein Accession#
NP_056511.2Gene Name
GPX7
Gene Alias
CL683, FLJ14777, GPX6, NPGPx
Gene Description
glutathione peroxidase 7
Gene Ontology
HyperlinkOther Designations
OTTHUMP00000010021|glutathione peroxidase 6|non-selenocysteine containing phospholipid hydroperoxide glutathione peroxidase
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Interactome
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Pathway
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Disease
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Publication Reference
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Glutathione-dependent and -independent oxidative stress-control mechanisms distinguish normal human mammary epithelial cell subsets.
Kannan N, Nguyen LV, Makarem M, Dong Y, Shih K, Eirew P, Raouf A, Emerman JT, Eaves CJ.
PNAS 2014 May; 111(21):7789.
Application:WB-Ce, Human, Human mammary cells.
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Identification of a novel putative non-selenocysteine containing phospholipid hydroperoxide glutathione peroxidase (NPGPx) essential for alleviating oxidative stress generated from polyunsaturated fatty acids in breast cancer cells.
Utomo A, Jiang X, Furuta S, Yun J, Levin DS, Wang YC, Desai KV, Green JE, Chen PL, Lee WH.
The Journal of Biological Chemistry 2004 Oct; 279(42):43522.
Application:IF, IP, WB, Human, Mouse, HS27 cells, MEFs.
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Glutathione-dependent and -independent oxidative stress-control mechanisms distinguish normal human mammary epithelial cell subsets.
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