PTCH1 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
PTCH1 polyclonal antibody (Cat # PAB7063) (1 ug/mL) staining of human brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunofluorescence
Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing Golgi/membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (5 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of PTCH1.
Immunogen
A synthetic peptide corresponding to internal region of human PTCH1.
Sequence
C-HPESRHHPPSNPRQQ
Host
Goat
Reactivity
Human, Mouse
Specificity
Western blot: Approx 150 kDa band observed in human brain lysates (calculated MW of 153.9 kDa according to NP_001077071.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Immunofluorescence (5 ug/mL)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide).
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
PTCH1 polyclonal antibody (Cat # PAB7063) (1 ug/mL) staining of human brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunocytochemistry
Immunofluorescence
Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing Golgi/membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (5 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).Enzyme-linked Immunoabsorbent Assay
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Gene Info — PTCH1
Entrez GeneID
5727Protein Accession#
NP_000255.2;NP_001077072.1;NP_001077071.1;NP_001077075.1Gene Name
PTCH1
Gene Alias
BCNS, FLJ26746, FLJ42602, HPE7, NBCCS, PTC, PTC1, PTCH, PTCH11
Gene Description
patched homolog 1 (Drosophila)
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the patched gene family. The encoded protein is the receptor for sonic hedgehog, a secreted molecule implicated in the formation of embryonic structures and in tumorigenesis, as well as the desert hedgehog and indian hedgehog proteins. This gene functions as a tumor suppressor. Mutations of this gene have been associated with basal cell nevus syndrome, esophageal squamous cell carcinoma, trichoepitheliomas, transitional cell carcinomas of the bladder, as well as holoprosencephaly. Alternative splicing results in multiple transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences and biological validity cannot be determined currently. [provided by RefSeq
Other Designations
OTTHUMP00000021709|OTTHUMP00000021710|PTCH protein +12b|PTCH protein +4'|PTCH protein -10|patched
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Interactome
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Pathway
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Disease
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Publication Reference
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Detecting tissue-specific alternative splicing and disease-associated aberrant splicing of the PTCH gene with exon junction microarrays.
Nagao K, Togawa N, Fujii K, Uchikawa H, Kohno Y, Yamada M, Miyashita T.
Human Molecular Genetics 2005 Nov; 14(22):3379.
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Detecting tissue-specific alternative splicing and disease-associated aberrant splicing of the PTCH gene with exon junction microarrays.
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