EZR polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
EZR polyclonal antibody (Cat # PAB7060) (1 ug/mL) staining of human placenta lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
EZR polyclonal antibody (Cat # PAB7060, 3.8 ug/mL) staining of paraffin embedded human small intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of EZR.
Immunogen
A synthetic peptide corresponding to human EZR.
Sequence
C-QDYEEKTKKAER
Host
Goat
Theoretical MW (kDa)
69.4
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:16000)
Western blot (1-3 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
EZR polyclonal antibody (Cat # PAB7060) (1 ug/mL) staining of human placenta lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
EZR polyclonal antibody (Cat # PAB7060, 3.8 ug/mL) staining of paraffin embedded human small intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — EZR
Entrez GeneID
7430Protein Accession#
NP_0.03370.2Gene Name
EZR
Gene Alias
CVIL, CVL, DKFZp762H157, FLJ26216, MGC1584, VIL2
Gene Description
ezrin
Omim ID
123900Gene Ontology
HyperlinkGene Summary
The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000017506|OTTHUMP00000017507|cytovillin 2|villin 2 (ezrin)
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Interactome
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Pathway
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Disease
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Publication Reference
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Crosstalk of protein clearance, inflammasome, and Ca2+ channels in retinal pigment epithelium derived from age-related macular degeneration patients.
Viivi Karema-Jokinen, Ali Koskela, Maria Hytti, Heidi Hongisto, Taina Viheriälä, Mikko Liukkonen, Tommi Torsti, Heli Skottman, Anu Kauppinen, Soile Nymark, Kai Kaarniranta.
The Journal of Biological Chemistry 2023 Jun; 299(6):104770.
Application：IF, Human, iPSC-derived retinal pigment epithelium cells.
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Ezrin directly interacts with the alpha1b-adrenergic receptor and plays a role in receptor recycling.
Stanasila L, Abuin L, Diviani D, Cotecchia S.
The Journal of Biological Chemistry 2006 Feb; 281(7):4354.
Application：WB-Tr, Human, HEK 293 cells.
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Crosstalk of protein clearance, inflammasome, and Ca2+ channels in retinal pigment epithelium derived from age-related macular degeneration patients.
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