SILV polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
SILV polyclonal antibody (Cat # PAB7056) (1 ug/mL) staining of human skin lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot (Transfected lysate)
HEK293 overexpressing human SILV and probed with SILV polyclonal antibody (Cat # PAB7056) (mock transfection in first lane), tested by Origene.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of SILV.
Immunogen
A synthetic peptide corresponding to human SILV.
Sequence
CPIGENSPLLSGQQ
Host
Goat
Theoretical MW (kDa)
70.3
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:16000)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
SILV polyclonal antibody (Cat # PAB7056) (1 ug/mL) staining of human skin lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Western Blot (Transfected lysate)
HEK293 overexpressing human SILV and probed with SILV polyclonal antibody (Cat # PAB7056) (mock transfection in first lane), tested by Origene.Enzyme-linked Immunoabsorbent Assay
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Gene Info — SILV
Entrez GeneID
6490Protein Accession#
NP_008859.1Gene Name
SILV
Gene Alias
D12S53E, ME20, PMEL, PMEL17, SI, SIL, gp100
Gene Description
silver homolog (mouse)
Omim ID
155550Gene Ontology
HyperlinkGene Summary
mouse
Other Designations
Melanocyte protein mel 17|Pmel 17|Silver, mouse, homolog of|melanosomal matrix protein17|silver (mouse homolog)-like|silver homolog
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Interactome
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Disease
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Publication Reference
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Functional amyloid formation within mammalian tissue.
Fowler DM, Koulov AV, Alory-Jost C, Marks MS, Balch WE, Kelly JW.
PLoS Biology 2006 Jan; 4(1):e6.
Application：IEM, IF, Bovine, Bovine RPE cells.
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Functional amyloid formation within mammalian tissue.
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