EBAG9 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of EBAG9.
Immunogen
A synthetic peptide corresponding to human EBAG9.
Sequence
C-KEAQRLMKKEQN
Host
Goat
Theoretical MW (kDa)
24.4
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:64000)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
EBAG9 polyclonal antibody (Cat # PAB7053) (0.2 ug/mL) staining of A-431 lysate (35 ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — EBAG9
Entrez GeneID
9166Protein Accession#
NP_004206.1Gene Name
EBAG9
Gene Alias
EB9, PDAF, RCAS1
Gene Description
estrogen receptor binding site associated, antigen, 9
Omim ID
605772Gene Ontology
HyperlinkGene Summary
This gene was identified as an estrogen-responsive gene. Regulation of transcription by estrogen is mediated by estrogen receptor which binds to the estrogen-responsive element (ERE) found in the 5'-flanking region of this gene. The encoded protein is a tumor-associated antigen that is expressed at high frequency in a variety of cancers. Two transcript variants differing in the 5' UTR, but encoding the same protein, have been identified for this gene. [provided by RefSeq
Other Designations
cancer associated surface antigen|estrogen receptor binding site associated antigen 9
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Interactome
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Publication Reference
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EBAG9 adds a new layer of control on large dense-core vesicle exocytosis via interaction with Snapin.
Ruder C, Reimer T, Delgado-Martinez I, Hermosilla R, Engelsberg A, Nehring R, Dorken B, Rehm A.
Molecular Biology of the Cell 2005 Mar; 16(3):1245.
Application:IF, WB-Tr, WB-Ce, Human, Rat, HEK 293, PC12 cells.
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EBAG9 adds a new layer of control on large dense-core vesicle exocytosis via interaction with Snapin.
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