Chat polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
The Chat polyclonal antibody (Cat # PAB7033) (0.2 ug/mL) staining of mouse brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of Chat.
Immunogen
A synthetic peptide corresponding to mouse Chat.
Sequence
C-KERARGPTKPSNLD
Host
Goat
Theoretical MW (kDa)
72.3
Reactivity
Mouse, Rat
Specificity
This antibody is NOT expected to cross-react to human CHAT.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western Blot (0.2-0.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
The Chat polyclonal antibody (Cat # PAB7033) (0.2 ug/mL) staining of mouse brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — Chat
Entrez GeneID
12647Protein Accession#
NP_034021.1Gene Name
Chat
Gene Alias
B230380D24Rik
Gene Description
choline acetyltransferase
Gene Ontology
HyperlinkOther Designations
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Publication Reference
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Choline acetyltransferase activity at different ages in brain of Ts65Dn mice, an animal model for Down's syndrome and related neurodegenerative diseases.
Contestabile A, Fila T, Bartesaghi R, Contestabile A, Ciani E.
Journal of Neurochemistry 2006 Apr; 97(2):515.
Application：ICC, IF, WB-Ti, Mouse, Mouse brain.
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Choline acetyltransferase activity at different ages in brain of Ts65Dn mice, an animal model for Down's syndrome and related neurodegenerative diseases.
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